Volume 23, Issue 2 (Scientific Journal of Hamadan University of Medical Sciences-Summer 2016)                   Avicenna J Clin Med 2016, 23(2): 134-140 | Back to browse issues page

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Sedighi F, Abbasali Pourkabir R, Maghsood A, Fallah M. Comparison of Therapeutic Effect of Anti-Cryptosporidium Nano-Nitazoxanide (NTZ) with Free form of this Drug in Neonatal Rat. Avicenna J Clin Med 2016; 23 (2) :134-140
URL: http://sjh.umsha.ac.ir/article-1-1174-en.html
Abstract:   (7822 Views)

Introduction & Objective: Cryptosporidiosis caused by Cryptosporidium, which is a protozoan parasite, has a worldwide distribution. The infection is through fecal-oral route, direct or indirect contact, food or water. The treatment of cryptosporidiosis is difficult and the anti-parasitic agents are not effective. The purpose of this study was encapsulation of nitazoxanide in solid lipid nano-particles (SLN) and investigation of its anti-Cryptosporidium effect and its comparison with free drug in the neonatal rat.    

Materials & Methods: Nitazoxanide was encapsulated by HPH method with 2 mg/Kg concentration in SLN nanoparticles. The oocysts were collected from calves and purified by sucrose floatation. A total of 72 Wistar neonatal rats were categorized in 6 groups of 12 rats including four infected groups treated by free drug, encapsulated nano drug, colloidal carriers without drug (SLN) and olive oil; an infected control group and a healthy control group that received PBS. 5 × 105 of oocyts inoculated orally into the sample groups. Finally, intestine of each rat was homogenized in PBS by rotor and the homogenized material was passed through a sieve. Then, floated oocysts in sucrose solution were counted by hemocytometer.

Results: Treatment by nitazoxanide significantly decreased the number of parasites in the treatment groups. This decrease at day 6 was more than day 3. Nano nitazoxanide had more effects on parasites than free drug. This difference at day 3 of treatment was not significant (p= 0.182) but at day 6 was statistically significant (P< 0.001).

Conclusion: Using nano-nitazoxanide could be a more effective way in the treatment of Cryptosporidium infections.

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Type of Study: Original | Subject: Other Clinical Specialties

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