Volume 17, Issue 2 (Scientific Journal of Hamadan University of Medical Sciences-Summer 2010)                   Avicenna J Clin Med 2010, 17(2): 24-31 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abnosi M H, Soleimani Mehranjani M, Momeni H, Mahdieh Najafabadi M, Shojafar E, Barati M. Effect of Sodium Arsenite on Rat Bone Marrow Mesenchymal Stem Cells: Cells Viability and Morphological Study. Avicenna J Clin Med. 2010; 17 (2) :24-31
URL: http://sjh.umsha.ac.ir/article-1-275-en.html
, abnosi2002@yahoo.com
Abstract:   (1455 Views)

Introduction & Objective: Sodium arsenite as an environmental pollutant being found in the air, water, and earth crust threats the human beings' health. The aim of this study was to investigate the effect of sodium arsenite on viability and morphology of mesenchymal stem cells in rat bone marrow.

Materials & Methods: In this exprimental study the cells were extracted in DMEM containing 15% FBS and Pen/Strep until the 3rd passage then treated with 0, 0.1, 0.5, 2.5, 12.5 and 20 µM of sodium arsenite for 12, 24, 36 and 48 hrs. Viability of the cells was carried out with trypan blue and MTT staining, then 0.1 µM and 36 hrs treatment was selected for further investigations. Morphology of the cells was studied using fluorescent dye (Hochest, propidium iodide and acridine orange as well as protein profile of the cells were studied using SDS-PAGE. Data was analyzed using one and two way ANOVA.

Results: Based on the two way ANOVA, cumulative effect of treatment time and used dosage caused highly significant reduction (p<0.001) in viability of rat bone marrow mesenchymal stem cells. One way ANOVA indicated that the viability of the cells reduced significantly (p<0.05) from 0.1 µM of sodium arsenite on wards in all the treatment time. Morphological changes including condensation and deformation of the nuclei, membrane disruption, and shrinkage of cytoplasm were also observed.

Conclusion: Sodium arsenite toxicity caused morphological and protein profile changes as well as dose and time dependent reduction in viability of rat bone marrow mesenchymal stem cells.

Full-Text [PDF 306 kb]   (457 Downloads)    
Type of Study: Original | Subject: Special

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2018 All Rights Reserved | Avicenna Journal of Clinical Medicine

Designed & Developed by : Yektaweb