Introduction & Objectives: This study was performed to determine the role of frozen section examination (FSE) of the cone specimen in the evaluation of the resection margin status and to rule out invasion in patients with high-grade cervical intraepithelial neoplasia.

Materials & Methods: Twenty patients with high grade intraepithelial neoplasia undergoing conization biopsy and frozen section examination were cross- sectionally studied from March 2008 until September 2009.The results of permanent paraffin sections were compared using FSE.

Results: In this cross-sectional study, 15 out of 20 cases (75%) showed the same results in frozen and permanent sections of cone biopsy specimen. Two patients out of the remaining 5 ones had higher grade of CIN in frozen section, 2 had lower grade and one was considered the same because CIN1 in FSE was normal in permanent sections.although The Paired Sample t-test showed no significant difference between the two groups of frozen and permanent section results (P-value=0.716, CI=95%).

Conclusion: Frozen section evaluation of cervical cone biopsy specimens in patients with CIN II-III is accurate, efficient, and cost-effective.