TY - JOUR T1 - Comparison of Two Different Disk Diffusion Agar Tests in Determination of Antibiotic Susceptibility for E-Coli Isolated from Urinary Tract Infection in Pediatrics TT - مقایسه دو روش دیسک دیفیوژن آگار با دیسک های ایرانی وخارجی در تعیین حساسیت آنتی بیوتیکی سویه های اشرشیا کلی جدا شده از کودکان مبتلا به عفونت ادراری JF - umsha JO - umsha VL - 17 IS - 1 UR - http://sjh.umsha.ac.ir/article-1-283-en.html Y1 - 2010 SP - 17 EP - 20 KW - Escherichia coli KW - Disk Diffusion Test KW - Microbial Sensitivity Tests KW - Urinary Tract Infections N2 - Introduction & Objective: Urinary Tract Infection (UTI) is one of the most common infections during childhood and E-Coli is the more predominant pathogen recovered in UTI. Disk Diffusion agar test is a method of choice because it is cost effective, simple, and now routinely used for detection of antibiotic susceptibility. A rapid increase in antibiotic resistance in our region made the authors to design a study to compare this traditional method with two different disk diffusion agar tests. Materials & Methods: Our study was conducted between 2009 and 2010 in Be’sat teaching hospital on 100 pediatric patients ranged 15 days to 13 years old with positive urine culture for E-coli. Antibiogram detection was performed by disk diffusion agar test with two different kits as Padtan-Teb (made in Iran) and Mast (made in the U.K.) for Co-trimoxazol, Amikacin, Ceftriaxone, Nalidixic Acid, Cefixime, and Nitrofurantoin. At last the data was analyzed by McNemar test. Results: Co-trimoxazol obtained the lowest (23% Padtan-Teb and 26% Mast) and Nitrofurantoin had the highest (86% Padtan-Teb and 97% Mast) sensitivity in the two methods which were used in our study. The results were statistically significant for Amikacin, Ceftriaxone, Cefixime, and Nitrofurantoin. The data was analyzed by Mc Memar test. Conclusion: According to our study the results of antibiotic susceptibility were more compatible with other non national Disk diffusion agar test and thus we recommend that our manufactures in Iran should increase the quality of their products. M3 ER -