RT - Journal Article T1 - Survey of Growth Hormone Effects on Methotrexate Side Effects in Rat Testis JF - umsha YR - 2007 JO - umsha VO - 14 IS - 3 UR - http://sjh.umsha.ac.ir/article-1-413-en.html SP - 40 EP - 47 K1 - Hormone K1 - Growth K1 - Methotrexate K1 - Rats K1 - Spermatogenesis K1 - Testis AB - Introduction & Objectives: Methotrexate (MTX) is a chemotherapeutic agent that used for the treatment of a variety of tumors and inflammatory diseases. The aim of this study was planed to see the role of human growth hormone (hGH) on testis recovery after treatment with MTX in rat. Materials & Methods: In this experimental study the fifty male wistar rat were selected and randomly divided into five groups control (n=10) and test (n=40). Hormone grope was received 0.3mg/kg hGH (IP) for 28 consequence day, drug group was received 1mg/kg MTX (IP) per week for four consequence week, protective group was received 0.3mg/kg hGH (IP) for 28 consequence day plus1mg/kg MTX (IP) per week for four consequence week and treatment group was received 1mg/kg MTX (IP) per week for two consequence week with 0.3mg/kg hGH (IP) for14 consequence day, however the control group just received vehicle (IP). In 14 and 28 day the testis tissue of rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. The statically analysis was ANOVA test. Results: This study was confirmed MTX had destructive effects in testis germinal cells and rate of spermatogenesis was decreased (P LA eng UL http://sjh.umsha.ac.ir/article-1-413-en.html M3 ER -