TY - JOUR T1 - Amygdala Centralis Cardiovascular Response to Renin Substrate Microinjection in Renovascular Hypertensive Rats TT - بررسی اثرات تزریق سوبسترای رنین در هسته آمیگدال مرکزی بر فشارخون شریانی و ضربان قلب در موش صحرایی مبتلا به پرفشاری خون کلیوی ـ عروقی JF - umsha JO - umsha VL - 13 IS - 4 UR - http://sjh.umsha.ac.ir/article-1-456-en.html Y1 - 2007 SP - 20 EP - 26 KW - Amygdala Centralis KW - Hypertension KW - Renin-Angiotensin System KW - Renin Substrate N2 - Introduction & Objectives: Previous studies have revealed that induction of two kidney-one clip (2K1C) Goldblatt hypertension in rats eliminates hypertensive response after inactivating of the Central Nucleus of the Amygdala (CA). The present study investigated the possibility of alteration in local Renin Angiotensin System (RAS) activity in CA after hypertension induction and evaluation of cardiovascular response to Renin Substrate (RS) microinjection into CA in Wistar rats. Materials & Methods: Clamps were placed on the left renal arteries to induce 2K1C Goldblatt hypertension. A similar surgery procedure without clipping was done in sham group. Rats were studied eight weeks after surgery. The Mean Arterial Blood Pressure (MAP) and heart rate (HR) were measured directly with indwelling arterial cannula under urethane anesthesia. Rats received bilateral cannulation of the CA for RS microinjection. After bilateral microinjection of consecutive doses of RS (0.5µL- 0.1, 1 & 10 µM) saline and pretreatment of losartan (10µM), saralasin(1nM) or captopril(10µM) before RS into CA, MAP and HR measured and compared between groups. Results: Bilateral microinjection of consecutive doses of RS   into CA increased MAP and HR significantly greater in hypertensive group in comparison with normotensive ones (P M3 ER -