AU - Soleimani Mehranjani, Malek AU - Mahdiyeh, Majid AU - Hoseini, Sedighe AU - Azimi, Atena Sadat TI - The Effect of Vitamin E on the In Vitro Differentiation of Adult Rat Bone Marrow Mesenchymal Stem Cells to Osteoblast During Sodium Arsenite Exposure PT - JOURNAL ARTICLE TA - umsha JN - umsha VO - 22 VI - 4 IP - 4 4099 - http://sjh.umsha.ac.ir/article-1-554-en.html 4100 - http://sjh.umsha.ac.ir/article-1-554-en.pdf SO - umsha 4 AB  - Introduction & Objective: Sodium arsenite disturbs the differentiation of adult rat bone marrow mesenchymal stem cells (rMSCs) to Osteoblast through oxidative stress. We aimed to investigate the preventive effect of vitamin E, a strong antioxidant, in sodium arsenite toxicity on rMSCs differentiation to osteoblast. Materials & Methods: rMSCs were cultured in Dulbecco’s Modified Eagles Medium containing 15% Fetal Bovine Serum and divided into: control, sodium arsenite (20 nM), vitamin E (50 µM) and sodium arsenite + vitamin E for 21 days in the osteogenic media containing 10% of fetal bovine serum. Cell viability, bone matrix mineralization, intercellular and extracellular calcium, alkaline phosphatase activity, DNA damage and cell morphological changes were evaluated. Data were analyzed using one-way ANOVA and Tukey's test and means were considered significantly different at P CP - IRAN IN - LG - eng PB - umsha PG - 276 PT - Original YR - 2016