2024-03-29T14:06:12+03:30
http://sjh.umsha.ac.ir/browse.php?mag_id=12&slc_lang=fa&sid=1
12-527
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Evaluation of Association Between Human Papilloma Virus and Squamous Cell Carcinoma of the Skin by Polymerase Chain Reaction Technique
Mojgan
Mokhtari
mokhtari@med.mui.ac.ir
Zahra
Bayat
Introduction & Objective : Squamous cell carcinoma (SCC) of the skin is one of the tumors arising from the epidermis, mucous membranes or anogenital region. This tumor has high rate of invasion and metastases . Regional lymph nodes metastases are reported. This tumor has high incidence in middle-aged and old persons. Human papilloma virus (HPV) has been detected in cutaneous SCC in some studies. Since the rate of recurrence and invasion of this tumor is high, finding association between SCC and HPV can help to apply antiviral treatment against this virus.
Materials & Methods : In this study 66 paraffin embedded skin SCC and their margins were selected and DNA isolation was performed by high pure product purification kit. By PCR technique , chain extension and primer annealing and denaturing was performed on DNA. Then PCR product was evaluated by electrophoresis with agar gel beside +/- control.
Results : 13 out of 66 (19.7%) SCC specimens and 2 out of margins (3%) were positive for HPV. Among the HPV positive cases, 86.7% were in the tumors and 13.3% were perilesional (P.value=0.0003).
Conclusion: We found a positive association between HPV and skin SCC. Therefore antiviral drugs application may be useful in prevention of the improvement , recurrence and invasion of skin SCC.
Carcinoma
Squamous Cell
Papilloma virus
Human
Polymerase Chain Reaction
2005
12
01
5
9
http://sjh.umsha.ac.ir/article-1-527-en.pdf
12-528
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Study on the Antidiabetic Effect of Carbon Tetrachloride Extract of Trigonella Foenum Seed in Streptozocin Induced Diabetic Rats
Saleh
Zahedi Asl
zahedi@erc.ac.ir
Behzad
Zaree
Introduction & Objective : Diabetes mellitus is a disease with a long history. Attempts to relieve the symptoms have not been totally successful. The seeds of Trigonella foenum have been shown to possess hypoglycemic properties. In this study hypoglycemic effect of the carbon tetra chloride (CCL4) extract of these seeds has been investigated.
Materials & Methods : Using Wistar rats, diabetes was induced by intravenous injections of 100 mg/kg of STZ in three doses divided over three consecutive days. The extract was prepared by the maceration method and administered orally in different doses. Blood samples were collected and serum glucose was measured by the O-toloidin method.
Results : The results indicated that the extract can reduce serum glucose in a dose dependent manner (r=0.44, P=0.00) and the maximum effect occurred with a dose of 4g/kg. With this dose, serum glucose concentration was reduced from 547±44 to 345±35 mg/dl (P=0.0000001).
Conclusion: The results of this study indicated that the CCL4 extract of trigonella foenum seeds have anti-diabetic effect, when it is introduced orally. The purification and formulation of its effective substance (s) for therapeutic purposes deserves consideration.
Carbon Tetrachloride
Diabetes Mellitus
Rats
Streptozocin
Trigonella Foenum
2005
12
01
10
15
http://sjh.umsha.ac.ir/article-1-528-en.pdf
12-529
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Determination of Prophylactic Calcium Gluconate Injection Efficacy in Prevention of Preterm Infants Hypocalcemia
Nasrollah
Pezeshki
pezeshki@umsha.ac.ir
Khosro
Mani Kashani
Introduction & Objective: Hypocalcemia is a common metabolic disorder during neonatal period that affects up to 30% of preterm infants. Hypocalcemia has significant importance because it exaggerate several serious neonatal problems such as intraventricular hemorrhage, and their long term complications such as mental retardation and physical disability.
Materials & Methods : We conducted a double blind randomized controlled clinical trial study of 80 preterm infants to examine the effect of calcium gluconate infusion to prevent neonatal hypocalcemia.These infants were randomly divided in two groups of 40 each. Over 60 minutes of birth, blood sample was taken and then were received an intravenous infusion of either calcium gluconate (100 mg/kg) or placebo (normal saline solution), and total serum calcium concentration was measured at 24, 48, and 72 hours after birth.
Results: After assessment of the results, 16 hypocalcemic infants were found in the group that received calcium gluconate and 22 instances in the control group.
Conclusion: We concluded that a single dose infusion of calcium gluconate in preterm infants isn;#39t effective in prevention of symptomatic or asymptomatic early neonatal hypocalcemia (even tough from clinical view is effective), as well as decreasing the duration of infant;#39s hospitalization.
Calcium Gluconate
Infant
Preterm
Hypocalcemia - prevention &
control
2005
12
01
16
19
http://sjh.umsha.ac.ir/article-1-529-en.pdf
12-530
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Frequency of Nasal Polyposis in Chronic Rhinosinusitis and Role of Endoscopic Examination in Correct Diagnosis
Farnaz
Hashemian
Farhad
Farahani
dr_f_farahani@yahoo.com
Introduction & Objective : Chronic rhinosinusitis (C.R.S.) is one of the most common diseases in the world. Polyposis is a complication of C.R.S., due to allergy or inflammation. The purpose of this study was detection of the incidence of polyposis in patients with C.R.S.
Materials & Methods : This study was carried out on 192 patients with C.R.S. who underwent functional endoscopic sinus surgery during 2000-2003 in Hamadan. All patients with C.R.S symptoms referred to ENT clinics were examined by otolaryngologist and after establishing diagnosis of C.R.S. they received medical treatment and after nose and para nasal sinuses CT scan, if there was indication, FESS was done. The patients who had polyps were followed up to one year, and the results analyzed with SPSS.
Results : According to the results, incidence of polyposis in 192 patients with C.R.S. was 40%, the sex distribution of the patients with polyposis was 60% in male and 40% in female. The age mean was 39.2 year. Involved sinuses in decreasing order of frequency was, anterior ethmoid , maxilla, Posterior ethmaid, sphenoid, sphenoid and frontal. 43% of the patients had history of allergy. Recurrence happened in 6.6% after one year follow up.
Conclusion : Because of disabling symptoms and severe complications of nasal polyposis, it is recommended more study in the future to find etiology and preventive ways for nasal polyposis in Hamadan.
Allergy
Endoscopic Surgery
Polyposis
Rhinitis
Sinusitis
2005
12
01
20
23
http://sjh.umsha.ac.ir/article-1-530-en.pdf
12-531
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Preparation of Somatic Antigen from Fusarium Solani for Serological Diagnosis of Fusariosis
Mohammad Reza
Aghamirian
aghamirian2001@yahoo.com
Farideh
Zaini
Introduction & Objective: Fusariosis is one of the most important systemic mycosis, often caused by Fusarium Solani and resist to antifungal drugs. The appropriate F. Solani antigen preparation could be useful in serodiagnosis of fusariosis.
Materials & Methods : The extraction procedure was preformed using F.Solani strain 7419 UAMH. The antigenic extract was obtained through grinding of fungal mass yielding from broth culture medium.
Results : Following fractionation of somatic antigen, two different component, that is, crude antigen as well as antigenic fractions (12,28) were collected.
The antigenic fractions in comparison with the crude antigen, demonstrated more effective antibody responses using ELISA method.
Conclusion: Availability of a suitable antigenic source could play a key role for serologic detecting of opportunistic fungal disease including fusariosis. Injection of this antigenic preparation in Rabbit resulted antibody response.
Fusarium solani
Fusariosis
Somatic Antigen
2005
12
01
24
28
http://sjh.umsha.ac.ir/article-1-531-en.pdf
12-532
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Treatment of Streptozotocin Induced Diabetic Male Rats by Immunoisolated Transplantation of Islet Cells
Azim
Akbarzadeh
azimakbarzadeh@pasteur.ac.ir
Shirin
Jamshidi
Behrokh
Farahmand
Behzad
Lame Rad
ُSeyyed Mohammad Ali
Mofidian
Introduction & Objective: Insulin injection is the main way to combat against insulin-dependent diabetes mellitus effects. Today in some laboratories in the world, the investigators are trying to find some treatments for this disease with insulin-secreting pancreatic islet cells transplantation. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression. This technique that is called immunoisolation is based on the principle that transplanted tissue is protected for the host immune system by an artificial or natural membrane.
Materials & Methods : Donor tissues in each step of work prepared from 6 adult male Wistar Rat with weight 250-300 grams (75-90 days). Transplantation were done in rats after 2-4 week induced diabetes with 60mg/ml streptozotocin injection via intravenous
Results: The data obtained from this study showed that islet cells can be enclosed in a semi permeable membrane that permits oxygen and nutrients to reach the islets, allowing insulin to be released into the blood stream and at the same time excluding potentially destructive immune cells and antibodies.
Conclusion: Testis subcutaneous and intrapretonea implantation of pure islet cells graft, that is a natural immunoisolation method, rapidly and permanently normalized the diabetic state of streptozocin-administered animals
Diabetes Mellitus
Immunoisolation
Islet Cells
Transplantation
2005
12
01
29
35
http://sjh.umsha.ac.ir/article-1-532-en.pdf
12-534
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Effect of Obstructive Cholestasis on Sperm Parameters in the Adult Male Rats
Ebrahim
Nasiri
ebrahimnasiri@yahoo.com
Ahmad Reza
Dehpour
Ali Reza
Zamani
Iraj
Amiri
Introduction & Objective: Obstructive cholestasis is associated with overproduction of endogenous opioids , nitric oxide and cytokins in the blood streams. These consequences may affect sex hormones since proper fertility will be resulted from physiological balance of sex hormones, we investigated the relationship between obstructive cholestasis and gonadotropins and sperm parameters in adult male rats.
Materials & Methods: To study this, we used three groups of animals: control (No-surgery), Sham (surgical control), and cholestatic (surgical ligation of the bile duct). After 3 weeks all animals were killed by ether, and serum concentrations of FSH and LH were determined by radioimmunoassay, sperm parameters were evaluated by light microscop.
Results: The findings of this study showed that LH and FSH levels decreased significantly in cholestatic compared to control and sham groups (p0.05).
Conclusion: These findings indicated that obstructive cholestasis lead to decrease in the levels of serum gonadotropins, but it has no significant effect on sperm parameters. We speculated that spermatogenesis, and sperm parameters were not dependent on gonadotropin hormones but other factors may involved.
Cholestasis
Gonadotropins
Rats
Sperm
2005
12
01
36
41
http://sjh.umsha.ac.ir/article-1-534-en.pdf
12-535
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Comparison of Face to Face Education with Other Methods to Pregnant Mothers in Increase Exclusive Breast Feeding
Mohammad Sadegh
Saba
dr_saba_ped@yahoo.com
Hasan
Bazmamoun
Zahra
Razavi
Introduction & Objective: There is a direct relationship between the education and exclusive breast-feeding. Most of the educational programs have given after child birth, based on " Rooming in plan”, but because of difficult situation and busy thoughts of mothers, they were not desirable way of education, therefore in this study the role of verbal & nonverbal education was evaluated on the rate of breast-feeding by mothers before delivery.
Materials & Methods: This is a randomized controlled-trial study on 108 pregnant women, 20-30 yrs old, paraone, without any systemic disease referring to Health Center of Hamadan. They were divided into two groups of 54 each , group ;Iota was given verbal education (lecture, movie, slide, question & answer) and group ;Iota;Iota nonverbal education (pamphlet of verbal abstracts). The two groups were followed up to 6 months after delivery in respect of restricted breast-feeding.
Results: The rate of restricted breast-feeding was 82.7% for mothers on verbal education and 56.5% for nonverbal ones. This difference of percentile rise in two group was statistically meaningful, which showed verbal education was more efficient on outcome of restricted breast-feeding.
Conclusion: We concluded that face to face education is more efficient compared to non-verbal education in increase exclusive breast feeding.
Breast Feeding
Breast Feeding
Restricted
Lactation
2005
12
01
42
47
http://sjh.umsha.ac.ir/article-1-535-en.pdf
12-536
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Typing of Typhoidal Salmonella Using Extraction of Water Soluble Whole Cell Proteins and Analysing by SDS-PAGE
Rasoul
Yousefi Mashouf
yousefimash@yahoo.com
Mohammad Taghi
Goodarzi
Saeed
Azadi
Introduction & Objective : Salmonella is one of the most important genus of Enterobacteriacea family. The aim of this study was typing of typhoidal Salmonella by SDS-PAGE and comparing the results with those of serotyping method.
Materials and Methods: In this study, 4 reference strains of Salmonella species, 5 reference strains of Enterobacteriacea family and 100 clinical isolates of Salmonella that were previously collected from laboratories of Hamadan medical centers were studied. Serotyping of strains were performed by Biomereux and Difco monovalent antisera. Whole-cell proteins of strains were also separated on 10% poly acrylamide gel. Gels were stained by Coomassie Brilliant Blue and analyzed by densitometry.
Results: Of 100 cases of Salmonella species, 43 cases (43%) were S. typhi, 20 cases (20%) were S. typhymurium, 12 cases (12%) were S. para typhi B, 10 cases (10%) were S. para typhi C, S. para typhi A 1 case (1%) and other cases were non-typhoidal Salmonella. The results of serotyping were compared with the results obtained by SDS-PAGE. Many protein bands from 220 KDa to 18.5 KDa were detected by SDS-PAGE and they were used to differentiate the strains. S. typhi serotypes were divided into 5 sub-species and S. para typhi B and C were divided each into 3 sub-species. Protein profiles of the reference strains of Salmonella were compared with protein profiles of Enterobacteriaceae species and showed some differences in major protein bands, however, they had a very similar protein band in 43 KDa area.
Conclusion: Since our data was able to divide Salmonella species to sub-types and differentiate them from Enterobacteriacea species, we concluded that analsying SDS-PAGE profile of water soluble whole-cell proteins can be used for typing of these organisms and it is comparble with serotyping, nevertheless, further researches are needed to establish SDS-PAGE method and to replace it with serotyping method.
Proteins
Whole Cell
Salmonella
Typing
2005
12
01
48
52
http://sjh.umsha.ac.ir/article-1-536-en.pdf
12-537
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
Descriptive Cross Sectional Study of Clinical Manifestation and MRI Finding in 60 Multiple Sclerosis Patients
Ali Akbar
Rezaie
aliakbarrezaei51@yahoo.com
Mohammad Saeed
Panahi
Introduction & Objective: Multiple Sclerosis (M.S) is common demyelinating diseases that is presented by inflammation and destruction of myelin of central nervous system such as different sizes of plaques. There are several methods for diagnosis of pathological findings in M.S., and one of them is MRI that is magnetic Resonance Imaging. In this study clinical manifestations and pathological findings of patient;#39s MRI in M.S. and also the relation between severity of signs and progression of MRI plaques were evaluated.
Materials & Methods : This was a descriptive cross sectional sudy that carried out on 60 patients with multiple sclerosis , in department of neurology of Hamadan Sina Hospital in 4 years (1999-2003). Clinical manifestation , age, sex, family History and past history of infectious and psychological diseases were evaluated. M.S characteristics like size, numbers and distribution were studied and collected data was analyzed with EPI6 software.
Results: There was a positive relationship between severity of clinical manifestations and low ages and history of several viral infections but there was not statistical relation between severity of manifestations and plaques criteria;#39s as their diameter, size and dispersion of plaques in MRI.
Conclusion: It was concluded that it is not possible to predict the severity of symptoms of MS according to the MRI results , solely , but study of both factors simultaneously is necessary in proper evaluation and diagnosis of the disease.
Clinical Manifestations
Magnetic Resonance Imaging
Multiple Sclerosis
Pathologic Plaques
2005
12
01
53
56
http://sjh.umsha.ac.ir/article-1-537-en.pdf
12-538
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
A Case Report of Acute Hemorrhagic Edema of Infancy
Mozhgan
Safari
dbazmamoun@yahoo.com
Hasan
Bazmamoun
Hesamoddin
Nabavizadeh
Introduction: Acute hemorrhagic edema of infancy is an acute and rare cutaneous disorder that affects children between 4 months to 2 years of age and characterized by cutaneous purpuric lesions with millimeters to centimeters diameter. Systemic involvement is rare. The disease is benign and spontaneously resolved in 1-3 weeks.
Case Report: Here we described a 22-months old girl with acute hemorrhagic edema of infancy who hospitalized in department of pediatric with the presence of erythematous-purpuric lesions localized on the face, ears and lower limbs that developed suddenly two days before hospitalization. Laboratory examination revealed including normal cell blood counts, serum complements, serum electrolytes and creatinin. ANA was negative. Coagulation tests were normal. ESR was 45 mm/h. Urine analysis and stool examination revealed no abnormal findings. Disease resolved spontaneously without any problem.
Conclusion: According to the contrast between the acuteness of the cutaneous sign, which are typical and unmistakable, and the general condition of the patient, which was good and the laboratory findings, our patient labeled as acute hemprrhagic edema of infancy.
Hemorrhagic Disease of Newborn
Infant
Newborn
Purpura
Schoenlein-Henoch
2005
12
01
57
59
http://sjh.umsha.ac.ir/article-1-538-en.pdf
12-539
2024-03-29
10.1002
Avicenna Journal of Clinical Medicine
Avicenna J Clin Med
2588-722X
2588-7238
10.52547/ajcm
2005
12
3
A Case Report of Squamous Cell Carcinoma of Gallbladder
Arash
Dehghan
Seyyed Mohammad
Hossienipanah
hosseinipanah@hotmail.com
Introduction : Epithelial cell carcinoma is the most common malignant neoplasm of gallbladder , but squamous cell carcinoma (SCC) is rare.
Case Report: Following the diagnosis of hydropsy of gallbladder in a sonography of a 60 years-old woman, cholesistectomy was performed. On macroscopic pathological examination, brownish deformed gallbladder was seen. After opening, on mucosal surface there was a cream, elastic polypoid tumoral lesion (2×1×0.5 cm). On microscopic evaluation, a pattern of pure and differentiated invasive Squamous Cell Carcinoma was diagnosed.
Conclusion: During the last twenty-two years, this has been the first report of SCC of gallbladder from province of Hamadan with incidence of 2.8% in Iran. This case can be used in hystogenesis of SCC of gallbladder.
Carcinoma
Squamous Cell
Gallbladder
2005
12
01
60
63
http://sjh.umsha.ac.ir/article-1-539-en.pdf