Volume 25, Issue 2 (Avicenna Journal of Clinical Medicine-Summer 2018)                   Avicenna J Clin Med 2018, 25(2): 112-120 | Back to browse issues page

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1- Associate Professor, Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran , mohammad.arabestani@gmail.com
Abstract:   (3635 Views)
Background and Objective: Secreted toxins of the burn wounds caused by Pseudomonas aeruginosa play an important role in spreading infection. The antibiotic resistance pattern may have an effect on the increased toxicity of this bacterium. Therefore, the aim of this study was to determine the relationship between the presence of P. aeruginosa toxins isolated from burn wounds and the antibiotic resistance pattern.
Materials and Methods: In this cross sectional-descriptive study, P. aeruginosa isolates were first isolated from the burn wound samples by using biochemical tests. The next step dealt with the investigation of antibiotic resistance pattern based on CLSI (Clinical and Laboratory Standards Institute). The process of identifying the genes responsible for producing toxin was performed by polymerase chain reaction method. Data analysis was performed in SPSS (version 16) using the Chi-square test.
Results: Out of 250 strains isolated from burn wounds, 63 isolates (25.2%) were obtained from P. aeruginosa. With regard to resistance, the obtained results revealed that Doripenem, Ertapenem, and Meropenem antibiotics had the highest frequency, whereas, the antibiotics of Piperacillin/Tazobactam and Piperacillin had the lowest frequency. Moreover, resistance to imipenem was observed to be semi-sensitive regarding the minimum inhibitory concentration in three isolates (76.4%). The exoS, toxA, exoT, exoY, and pvdA genes were observed in 40 (63.49%), 31(49.2%), 39 (61.9%), 56 (88.88%), and 50 (79.36%) isolates, respectively. In addition, there was a significant correlation between the antibiotic resistance pattern and distribution of toxin genes (P <0.05).
Conclusion: According to the obtained results, it can be concluded that the antimicrobial resistance pattern could play an important role in the distribution of P. aeruginosa toxin genes isolated from burn wounds.
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Type of Study: Original | Subject: Other Clinical Specialties

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