Volume 10, Issue 1 (Scientific Journal of Hamadan University of Medical Sciences-Spring 2003)                   Avicenna J Clin Med 2003, 10(1): 17-24 | Back to browse issues page

XML Persian Abstract Print

Abstract:   (9835 Views)

Recent studies in amnion and chorion trophoblasts have shown that glucocorticoids (GC) increase and decrease expression and activity of PGHS and PGDH enzymes respectively. However, the effects of GC on the expression of these enzymes in placental cells (PC) is unknown.

          Human placenta were obtained from uncomplicated term pregnancies after elective cesarian section delivery. The dispresed cells were filtered and loaded on to a continous percoll gradient (5-70%) and after 72 hrs incubation of primary culture cell medium were washed and changed and then treated for 24 hrs in the presence or absence of arachidonic acid (5mM), plus various
   combinations of meloxicam (MEL , 1
mM), indomethacin (Ind , 1mM) dexamethasone (DEX , 1mM) and sulphasalazine (SFZ , 1mM). fter incubation period cell mediums were collected for determination of PGE2 by radioimmunoassay. One-way ANOVA test and student’s t-test were used to assess statistical differences and ststistical significances were set at P<0.05.

          Dexamethasone produced a significant , almost doubling of PGE2 output , but this was not altered further by Mel. In the presece of SFZ alone , there was a significant approximate doubling increase in the output of PGE(P<0.05) and this increase further in the presence of dexamethasone that increase was reduced by further addition of Mel (P<0.05) . Indomethacine treatment significantly reduced stimulation of PGE2 output seen in the presence of SFZ , or
   the further increase seen in cells treated with SFZ plus dexamethasone.

          We conclude that basal PGEoutput by term human PC likely depends on the activity of PGHS-1 not PGHS-2 .The effects of SFZ show the importance of endogenous PGDH in regulating PGE2 output , and interactions with SFZ , DEX and MEL suggest that GC stimulated output of PGEby PC may be attributable to both up regulation of PGHS and decrease activity of PGDH.

Full-Text [PDF 454 kb]   (5769 Downloads)    
Type of Study: Original | Subject: Other Clinical Specialties

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.