Aspergillosis is an important systemic fungal infection with various clinical
   forms and its clinical and mycological diagnosis is difficult and
   compelicated but its serodiagnosis is simple and useful for some clinical
   forms (aspergillomma and allergic aspergillosis). This study was performed for
   preparing metabolic antigen and related antiserum of the most important
   agent of aspergillosis, “Aspergillus fumigatus", for using in serodiagnosing the
   disease.

          The fungus was isolated from the patients, and cultured in glucose
   peptone broth for 5 weeks. After removing the fungal mass, the secretary
   (metabolic) antigens was isolated and processed and injected to rabbit.
   Antisera were evaluated by double- diffusion (DD).

          Prepared antigen was able to immunize rabbits and reacted with rabbit
   and patient’s sera in immunodiffusion. Number and pattern of the bands
   was comparable with standard antigen and antiserum.

          After standardization, this antigen, it can be a useful reagent to
   serodiagnosis of aspergillosis by lower cost and more accesibility.