fa بیان متضاد ژن‌های نیدوژن-1 و Cdc42 در ایجاد سیروز کبدی بیماران مبتلا به کبد چرب غیر الکلی بيوشيمي باليني Clinical Biochemistry پژوهشي Original <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">سابقه و هدف:</span></span></span></span></span></span></b>&nbsp;<span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">دخالت ژن‌های نیدوژن-1 و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">به عنوان ژن‌های موثر در متابولیسم ماتریکس خارج سلولی در ایجاد فیبروز بافت‌های مختلف اثبات شده است. در مطالعه حاضر بیان ژن</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">های نیدوژن-1 و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">در بافت کبد بیماران مبتلا به سیروز، بیماران با استئاتوز ساده غیر الکلی و افراد کنترل بررسی شده است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.15pt">مواد و روش‌‌ها</span></span></span></span></span></span></b><b><span b="" lang="AR-SA" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.15pt">:</span></span></span></span></span></b><b>&nbsp;</b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه مورد - شاهد</span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">ی</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> 38 نمونه بافتی در </span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">سه گروه</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> سیروز (24 نمونه)، استئاتوز ساده (6 نمونه) و گروه کنترل (8 نمونه) جمع‌آوری و بررسی شده است. بیان ژن‌های نیدوژن-1 و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">با استفاده از روش </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">RT</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">-</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">qPCR</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">بررسی شده است.</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">یافته‌ها</span></span></span></span></span></span></b><b><span b="" lang="AR-SA" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">:</span></span></span></span></span></b>&nbsp;<span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">میزان بیان ژن‌های نیدوژن-1 و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">در بافت کبد با سیروز، نسبت به بافت کنترل کاهش معنادار داشته است (به ترتیب 0/008 و 0/001</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">). ژن نیدوژن-1 در بافت کبد با سیروز </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(</span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Nonalcoholic steatohepatitis</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> نسبت به گروه کنترل دارای کمترین بیان بوده و بیان این ژن در بافت کبد با استئاتوز ساده بین دو گروه سیروز </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">و کنترل قرار داشته است، اما بیان ژن </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">در بافت با استئاتوز ساده به طور معناداری کمتر از بیان آن در بافت کبد با سیروز </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">بوده است (0/043</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P=</span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">نتیجه‌گیری:</span></span></span></span></span></span></b><b>&nbsp;</b><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">بیان ژن‌های نیدوژن-1 و </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">در سیروز کاهش می‌یابند؛ ولی بیان متفاوتی بین شرایط استئاتوز و سیروز </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">دارند؛ بنابراین، نقش ضد فیبروزی نیدوژن-1 و نیز مشارکت </span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Cdc42</span></span> <span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">در تشکیل فیبروز امکان‌پذیر است.&nbsp;&nbsp;&nbsp;</span></span></span></span></span></span></span> <span style="font-family:Tahoma;"><span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span ms="" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Background and Objective:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:115%">The roles of Nidogen-1 and Cdc42 genes as significant factors in the metabolism of the extracellular matrix have been demonstrated in the fibrosis of various tissues. This study investigates the gene expressions of Nidogen-1 and Cdc42 in the liver tissue of patients with cirrhosis, as well as in patients with simple non-alcoholic steatosis and control subjects.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span ms="" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="color:#274cc3">Materials and Methods:</span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:115%">In this case-control study, 38 tissue samples were collected and analyzed across three groups: cirrhosis (24 samples), simple steatosis (6 samples), and the control group (8 samples). The expression of Nidogen-1 and Cdc42 genes was investigated using RT-qPCR.</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span ms="" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Results:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:115%">The gene expressions of Nidogen-1 and Cdc42 in tissues affected by cirrhosis were significantly lower compared to the control group (P=0.001 and P=0.008, respectively). The Nidogen-1 gene exhibited the lowest expression in tissues with Nonalcoholic steatohepatitis (NASH) cirrhosis when compared to the control group, while its expression in tissues with simple steatosis fell between that of the NASH cirrhosis and control groups. Conversely, the expression of the Cdc42 gene in tissues with simple steatosis was significantly lower than in liver tissues with NASH cirrhosis (P=0.043).</span></span></span></span></span></span></span><br> <b><span style="font-size:9.0pt"><span style="color:#274cc3"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span style="color:black">The gene expressions of Nidogen-1 and Cdc42 decrease in cirrhosis; however, they exhibit different levels between steatosis and NASH cirrhosis. Therefore, the anti-fibrotic role of Nidogen-1 and the involvement of Cdc42 in fibrosis formation are plausible</span></span></span>  استئاتوز ساده, سی‌دی‌سی 42, سیروز, نیدوژن ​​​​​​​ Cdc42, Cirrhosis, Nidogen, Simple Steatosis 197 205 http://sjh.umsha.ac.ir/browse.php?a_code=A-12-2752-2&slc_lang=fa&sid=1 Zahra Ali زهرا علی 100319475328460023450 100319475328460023450 No Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی همدان، همدان، ایران Amir Mohammad Zargar امیرمحمد زرگر 100319475328460023451 100319475328460023451 No Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran کمیته تحقیقات و فناوری دانشجویی، دانشگاه علوم پزشکی همدان، همدان، ایران Sina Mohagheghi سینا محققی amr.mohaghegh@yahoo.com 100319475328460023452 100319475328460023452 Yes Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی همدان، همدان، ایران