Avicenna Journal of Clinical Medicine
مجله پزشكي باليني ابن سينا
Avicenna J Clin Med
Medical Sciences
http://sjh.umsha.ac.ir
1
admin
2588-722X
2588-7238
-
10.53208/ajcm
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fa
jalali
1404
6
1
gregorian
2025
9
1
32
2
online
1
fulltext
fa
کاهش بیان ژن سرکوبگر تومور PTEN در بیماران مبتلا به عفونت مزمن ویروس هپاتیت B
Down-regulation of Tumor Suppressor Gene PTEN in Patients with Chronic Hepatitis B Virus Infection
بيماريهاي عفوني
Infectious Diseases
پژوهشي
Original
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.1pt">سابقه و هدف:</span></span></span></span></span></span></b> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">عفونت مزمن با ویروس هپاتیت</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(HBV) B </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">یکی از مهمترین عوامل خطر در ایجاد کارسینوم هپاتوسلولار</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(HCC)</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">به شمار میرود. </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">کاهش</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">یا سرکوب بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTEN</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">به ایجاد و پیشرفت </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HCC</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">، به ویژه در زمینه عفونت مزمن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HBV</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">، کمک میکند. هدف از این مطالعه، تعیین میزان بیان ژن</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTEN </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در بیماران مبتلا به</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HBV </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">مزمن در مقایسه با افراد سالم بود</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.15pt">مواد و روشها</span></span></span></span></span></span></b><b><span b="" lang="AR-SA" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.15pt">:</span></span></span></span></span></b><b> </b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">این مطالعه از نوع مورد-شاهدی بود که در آن </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۵۰</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بیمار مبتلا به عفونت مزمن</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HBV </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۵۰</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">فرد سالم وارد مطالعه شدند. استخراج</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RNA </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">از خون محیطی انجام شد و پس از سنتز </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cDNA</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">، میزان بیان ژن</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTEN </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">نسبت به ژن مرجع</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GAPDH </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با استفاده از روش</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">-</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Time PCR </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">مبتنی بر</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SYBR Green </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و با بهرهگیری از روش</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">2^-ΔΔCt </span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">سنجیده شد. تحلیل آماری با استفاده از نرمافزار</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">نسخه </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۲۶</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">انجام گرفت و سطح معناداری کمتر از </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">0/05</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در نظر گرفته شد</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">یافتهها</span></span></span></span></span></span></b><b><span b="" lang="AR-SA" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">:</span></span></span></span></span></b><b> </b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بیان ژن</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTEN </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در گروه بیمار بهطور معناداری کمتر از گروه کنترل بود</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">)</span></span> 0<span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">/74</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">, </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">2^-ΔΔCt </span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">= </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">0/0001</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">.(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><</span></span><span style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> همچنین با افزایش سن، کاهش بیشتری در میزان بیان</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTEN </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">مشاهده شد. این یافتهها حاکی از آن است که عفونت مزمن با</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HBV</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">میتواند بهمرور زمان موجب مهار بیان ژنهای سرکوبگر تومور گردد</span></span><span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#274cc3"><span style="position:relative"><span style="top:-1.0pt"><span style="letter-spacing:.2pt">نتیجهگیری: </span></span></span></span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج بهدستآمده، اهمیت</span></span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PTEN </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">را بهعنوان یک نشانگر زیستی احتمالی در پیشرفت بیماری به سمت سرطان کبد برجسته مینماید. از این رو، تحقیقات بیشتر با حجم نمونه بزرگتر و بررسی مکانیسمهای مولکولی دخیل در تنظیم بیان</span></span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PTEN </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">ضروری به نظر میرسد</span></span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Background and Objective:</span></span></span></span></b> <span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">Chronic Hepatitis B virus (HBV) infection is a major global health concern and a leading cause of hepatocellular carcinoma (HCC). Loss or suppression of PTEN expression contributes to the development and progression of HCC, particularly in the context of chronic HBV infection. The present study aimed to evaluate PTEN gene expression in patients with chronic HBV infection compared to healthy individuals.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3">Materials and Methods:</span></span></span></b> <span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">The present case-control study included 50 patients with chronic HBV infection and 50 healthy controls. Peripheral blood samples were collected, and total RNA was extracted and converted to cDNA. Gene expression of PTEN relative to GAPDH was assessed by SYBR Green-based Real-Time PCR using the 2-</span></span><span lang="X-NONE" style="font-size:9.0pt"><span cambria="" style="font-family:">ΔΔ</span></span><span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">Ct method. Statistical analysis was performed using the SPSS (version 26) software, with a significance set at P<0.05.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:.15pt">Results:</span></span></span></span></b> <span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">The PTEN gene expression was significantly down-regulated in the HBV-infected group compared to controls (2-</span></span><span lang="X-NONE" style="font-size:9.0pt"><span cambria="" style="font-family:">ΔΔ</span></span><span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">Ct = 0.074, P<0.0001). The </span></span><span lang="X-NONE" style="font-size:9.0pt"><span cambria="" style="font-family:">Δ</span></span><span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">Ct value of PTEN was significantly higher in the patient group, indicating reduced transcript levels. An age-related decline in PTEN expression was also observed among HBV patients.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span ms="" style="font-family:" trebuchet=""><span style="color:black"><b><span style="font-size:9.0pt"><span style="font-family:Optima"><span style="color:#274cc3"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></span></b> <span lang="X-NONE" style="font-size:9.0pt"><span style="font-family:Optima">The results highlight the importance of PTEN as a potential biomarker in the progression of the disease to liver cancer. Therefore, further research with a larger sample size and investigation of the molecular mechanisms involved in regulating PTEN expression seems necessary.</span></span></span></span></span></span></span></span><br>
بیان ژن, فرار از سیستم ایمنی, کارسینوم هپاتوسلولار, هپاتیت B مزمن
Chronic Hepatitis B, Gene Expression, Hepatocellular Carcinoma, Immune Evasion
80
86
http://sjh.umsha.ac.ir/browse.php?a_code=A-11-2976-2&slc_lang=fa&sid=1
Somayeh
Shokri
سمیه
شکری
100319475328460023739
100319475328460023739
No
Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran
مرکز تحقیقات بیماریهای عفونی، پژوهشکده علوم بالینی ابنسینا، دانشگاه علوم پزشکی همدان، همدان، ایران
Farid
Azizi Jalilian
فرید
عزیزی جلیلیان
100319475328460023740
100319475328460023740
No
Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran
مرکز تحقیقات پزشکی مولکولی، پژوهشکده سرطان، دانشگاه علوم پزشکی همدان، همدان، ایران
Razieh
Amini
راضیه
امینی
100319475328460023741
100319475328460023741
No
Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran
مرکز تحقیقات پزشکی مولکولی، پژوهشکده سرطان، دانشگاه علوم پزشکی همدان، همدان، ایران
Ali
Shojaeian
علی
شجاعیان
100319475328460023742
100319475328460023742
No
Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran
مرکز تحقیقات پزشکی مولکولی، پژوهشکده سرطان، دانشگاه علوم پزشکی همدان، همدان، ایران
Fatemeh
Torkaman Asadi
فاطمه
ترکمان اسدی
100319475328460023743
100319475328460023743
No
Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran
مرکز تحقیقات بیماریهای عفونی، پژوهشکده علوم بالینی ابنسینا، دانشگاه علوم پزشکی همدان، همدان، ایران
Shahab
Mahmoudvand
شهاب
محمودوند
Mahmoudvandsh100@gmail.com
100319475328460023744
100319475328460023744
Yes
Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran
مرکز تحقیقات بیماریهای عفونی، پژوهشکده علوم بالینی ابنسینا، دانشگاه علوم پزشکی همدان، همدان، ایران