Introduction & Objectives: Methotrexate (MTX) is a chemotherapeutic agent that used for the treatment of a variety of tumors and inflammatory diseases. The aim of this study was planed to see the role of human growth hormone (hGH) on testis recovery after treatment with MTX in rat.

Materials & Methods: In this experimental study the fifty male wistar rat were selected and randomly divided into five groups control (n=10) and test (n=40). Hormone grope was received 0.3mg/kg hGH (IP) for 28 consequence day, drug group was received 1mg/kg MTX (IP) per week for four consequence week, protective group was received 0.3mg/kg hGH (IP) for 28 consequence day plus1mg/kg MTX (IP) per week for four consequence week and treatment group was received 1mg/kg MTX (IP) per week for two consequence week with 0.3mg/kg hGH (IP) for14 consequence day, however the control group just received vehicle (IP). In 14 and 28 day the testis tissue of rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. The statically analysis was ANOVA test.

Results: This study was confirmed MTX had destructive effects in testis germinal cells and rate of spermatogenesis was decreased (P<0.05) as compared with control group, and HGh had treatment effects in degenerative seminiferous epithelium and improve spermatogenesis (P<0.05) as compared in other groups.

Conclusion: Since in our study hGH had recovery effect on spermatogenesis cycle after treatment with MTX, and it can not have useful effect if only prescript in chemotherapy period than consequently hGH inducement is suitable for sperm health ability parameters after chemotherapy in patient.