Volume 14, Issue 1 (Scientific Journal of Hamadan University of Medical Sciences-Spring 2007)                   Avicenna J Clin Med 2007, 14(1): 44-50 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khodadadi I, Thumser A. Different Responses of Cardiac Cells to Saturated and Unsaturated Fatty Acids. Avicenna J Clin Med 2007; 14 (1) :44-50
URL: http://sjh.umsha.ac.ir/article-1-447-en.html
1- , khodadadi@umsha.ac.ir
Abstract:   (3587 Views)

Introduction & Objective: The link between dietary fat and coronary heart disease has attracted much attention since the effect of long?chain fatty acids (LCFA) on gene transcription has been established, which in part, these effects can be explained by the regulation of gene transcription. In this study, the P19CL6 cardiac cell?line was targeted for the investigation of (i) the effects of long?chain fatty acids (LCFA) and clofibrate on mRNA levels of specific lipid metabolism?related genes, such as heart?type fatty acid?binding protein (H?FABP) and peroxisome proliferator?activated receptors (PPARa,b,g) in the P19CL6 cell?line, and (ii) to determine the effects of LCFAs and clofibrate on global transcriptome levels, using cDNA microarray analysis.

Materials & Methods: After culturing P19CL6 cells with LCFAs or clofibrate, the total?RNA was extracted and expression levels of H-FABP, PPARa, PPARb, and PPARg genes were determined by RT?PCR. In addition, microarray analysis was used to compare global transcriptome profiles in P19CL6 cells cultured with different LCFAs or clofibrate.

Results: LCFAs significantly increased the abundance of PPARa and PPARg. Moreover, microarray analysis showed the effects of linoleic and a?linolenic acids and clofibrate were similar but differed from those of palmitic and oleic acids..

Conclusion: These findings show cellular responses to polyunsaturated fatty acids differ from those observed with saturated and monounsaturated fatty acids.

Full-Text [PDF 246 kb]   (1249 Downloads)    
Type of Study: Original | Subject: Other Clinical Specialties

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Avicenna Journal of Clinical Medicine

Designed & Developed by : Yektaweb