Volume 23, Issue 1 (Scientific Journal of Hamadan University of Medical Sciences-Spring 2016)                   Avicenna J Clin Med 2016, 23(1): 49-56 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bidgoli M, Amiri I, Soheilifar S, Hoshyar E. In vitro Evaluation of the Effect of Melatonin on Osteogenic Differen-tiation of PDL Mesenchymal Stem Cells Isolated from Chronic Perio-dontitis Affected Teeth. Avicenna J Clin Med 2016; 23 (1) :49-56
URL: http://sjh.umsha.ac.ir/article-1-860-en.html
Abstract:   (4257 Views)

Introduction & Objective: In recent years stem cells have been evaluated for regenerating lost tissues. Therefore, many studies aimed to assess isolation of these cells from different origins, and culture them. The purpose of this study is to evaluate the osteogenic differentiation of stem cells derived from periodontal ligament of periodontitis affected teeth in melatonin contained media.

Materials & Methods: In this experimental-laboratory study after tooth extraction and isolating the apical segment of the roots, the periodontal ligament tissue was removed. Cells were expanded and after the third passage; flow cytometery analysis was performed to evaluate the surface markers (CD 105, CD146, CD90, CD 45, CD31, CD34, CD106 and CD 73). Adherent cell layer was used for osteogenic differentiation in melatonin contained osteogenic media. Then, osteogenic differentiation was evaluated using alizarin red staining, alkaline phosphatase and calcium content tests were performed.

Results: Quantitative analysis of alizarin red staining on the 28th day demonstrated that mineralized nodule formation in the group supplemented with melatonin was higher than the control group. Results from alkaline phosphatase activity test on the 7th, 14th and 21st days demonstrated that, this activity was higher in the group supplemented with melatonin. Also, the amount of calcium on the 7th, 14th, 21st and 28th days, in melatonin group, was higher than the control group.

Conclusion: Melatonin (50 µM) may be beneficial for differentiation of PDL stem cells into osteoblast.

 (Sci J Hamadan Univ Med Sci 2016; 23 (1):49-56)

Full-Text [PDF 397 kb]   (1929 Downloads)    
Type of Study: Original | Subject: Other Clinical Specialties

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Avicenna Journal of Clinical Medicine

Designed & Developed by : Yektaweb