Volume 25, Issue 4 (Avicenna Journal of Clinical Medicine-Winter 2019)                   Avicenna J Clin Med 2019, 25(4): 207-214 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirza Aghasi A, Ghorbian S. Evaluation of the PRSS8 Gene Methylation Pattern in Patients with Esophageal Squamous Cell Carcinoma. Avicenna J Clin Med 2019; 25 (4) :207-214
URL: http://sjh.umsha.ac.ir/article-1-1825-en.html
1- , s_ghorbian@iau-ahar.ac.ir
Abstract:   (3336 Views)
Background and Objective: Esophageal cancer is the seventh cause of cancer deaths in the worldwide. Epigenetic changes including methylation of genes promoter can play an important role in the pathogenesis of esophageal squamous cell (ESCC). The aim of this investigation was to determine the PRSS8 gene methylation pattern in patients with ESCC.
Materials and Methods: This case-control study was conducted on 150 paraffin blocks, including 75 paraffin blocks of cancerous tissues and 75 non-cancerous tissues paraffin blocks in ESCC patients. The Blocks in this study were from the archives of Noor-e-Nejat and Tabriz International Hospitals during 2013-2017. After DNA extraction and treatment with sodium metabisulfite, the PRSS8 gene methylation promoter was evaluated using High Resolution Melting (HRM) method.
Results: The findings revealed that there was a significant difference between the cancerous tissues and non-cancerous tissues regarding the level of PRSS8 gene methylation (P=0.026). In addition, the relationship between PRSS8 gene methylation and metastasis revealed no significant change in terms of clinicopathological parameters (P=0.007).
Conclusion: The present investigation showed that the PRSS8 gene promoter hypermethylation can play a critical role in the carcinogenesis and metastasis of ESCC
Full-Text [PDF 407 kb]   (1378 Downloads)    
Type of Study: Original | Subject: Genetics

References
1. Siegel R, Naishadham D, Jemal A. Cancer statistics for Hispanics/Latinos, 2012. CA Cancer J Clin. 2012;62(5):283-98. PMID: 22987332 DOI: 10.3322/caac.21153
2. Ohashi S, Miyamoto S, Kikuchi O, Goto T, Amanuma Y, Muto M. Recent advances from basic and clinical studies of esophageal squamous cell carcinoma. Gastroenterology. 2015;149(7):1700-15. PMID: 26376349 DOI: 10.1053/j. gastro.2015.08.054
3. Hosseintabar Marzoni AS, Moghimbeigi A, Faradmal J. Gastric and esophageal cancers incidence mapping in Golestan Province, Iran: using bayesian-gibbs sampling. Osong Public Health Res Perspect. 2015; 6(2):100-5. PMID: 25938019 DOI: 10.1016/j.phrp.2015.01.004
4. Ghorbian S, Ardekani AM. Non-invasive detection of esophageal cancer using genetic changes in circulating cell-free DNA. Avicenna J Med Biotechnol. 2012;4(1):3-13. PMID: 23407878
5. Jones PA, Baylin SB. The fundamental role of epigenetic events in cancer. Nat Rev Genet. 2002;3(6):415-28. PMID: 12042769 DOI: 10.1038/nrg816
6. Yu JX, Chao L, Ward DC, Chao J. Structure and chromosomal localization of the human prostasin (PRSS8) gene. Genomics. 1996;32(3):334-40. PMID: 8838796 DOI: 10.1006/geno.1996.0127
7. Yu JX, Chao L, Chao J. Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue distribution, and localization in prostate gland. J Biol Chem. 1994;269(29):18843-8. PMID: 8034638
8. Hooper JD, Bowen N, Marshall H, Cullen LM, Sood R, Daniels R, et al. Localization, expression and genomic structure of the gene encoding the human serine protease testisin. Biochem Biophys Acta. 2000;1492(1):63-71. PMID: 11004480
9. Sarojini S, Tamir A, Lim H, Li S, Zhang S, Goy A, et al. Early detection biomarkers for ovarian cancer. J Oncol. 2012; 2012:709049. PMID: 23319948 DOI: 10.1155/2012/709049
10. Takahashi S, Suzuki S, Inaguma S, Ikeda Y, Cho YM, Hayashi N, et al. Down-regulated expression of prostasin in high-grade or hormone-refractory human prostate cancers. Prostate. 2003;54(3):187-93. PMID: 12518323 DOI: 10.1002/pros.10178
11. Chen LM, Chai KX. Prostasin serine protease inhibits breast cancer invasiveness and is transcriptionally regulated by promoter DNA methylation. Int J Cancer. 2002;97(3):323-9. PMID: 11774283
12. Chen LM, Verity NJ, Chai KX. Loss of prostasin (PRSS8) in human bladder transitional cell carcinoma cell lines is associated with epithelial-mesenchymal transition (EMT). BMC Cancer. 2009;9:377. PMID: 19849847 DOI: 10.1186/1471-2407-9-377
13. Sakashita K, Mimori K, Tanaka F, Tahara K, Inoue H, Sawada T, et al. Clinical significance of low expression of prostasin mRNA in human gastric cancer. J Surg Oncol. 2008;98(7):559-64. PMID: 18932242 DOI: 10.1002/ jso.21158
14. Bao Y, Wang Q, Guo Y, Chen Z, Li K, Yang Y, et al. PRSS8 methylation and its significance in esophageal squamous cell carcinoma. Oncotarget. 2016;7(19):28540-55. PMID: 27081034 DOI: 10.18632/oncotarget.8677
15. Bedi U, Mishra VK, Wasilewski D, Scheel C, Johnsen SA. Epigenetic plasticity: a central regulator of epithelial-to-mesenchymal transition in cancer. Oncotarget. 2014; 5(8):2016-29. PMID: 24840099 DOI: 10.18632/oncotarget. 1875
16. Uchimura K, Hayata M, Mizumoto T, Miyasato Y, Kakizoe Y, Morinaga J, et al. The serine protease prostasin regulates hepatic insulin sensitivity by modulating TLR4 signalling. Nat Commun. 2014;5:3428. PMID: 24614850 DOI: 10.1038/ ncomms4428
17. Uchimura K, Kitamura K. Prostasin (PRSS8) as a new pathological factor. Nihon Rinsho. 2015;73(3):523-33. PMID: 25812384
18. Keppner A, Malsure S, Nobile A, Auberson M, Bonny O, Hummler E. Altered prostasin (CAP1/Prss8) expression favors inflammation and tissue remodeling in DSS-induced colitis. Inflamm Bowel Dis. 2016;22(12):2824-39. PMID: 27755216 DOI: 10.1097/MIB.0000000000000940
19. Dvojakovska S, Popovic-Monevska D, Grcev A, Pancevski G, Benedetti A, Popovski V, et al. Promotor hypermethylated genes: prospective diagnostic biomarkers in oral cancerogenesis. J Craniomaxillofac Surg. 2018;46(10):1737-40. PMID: 30100382 DOI: 10.1016/j.jcms.2018.07.019
20. Bao Y, Li K, Guo Y, Wang Q, Li Z, Yang Y, et al. Tumor suppressor PRSS8 targets Sphk1/S1P/Stat3/Akt signaling in colorectal cancer. Oncotarget. 2016;7(18):26780-92. PMID: 27050145 DOI: 10.18632/oncotarget.8511
21. Yan BX, Ma JX, Zhang J, Guo Y, Mueller MD, Remick SC, et al. Prostasin may contribute to chemoresistance, repress cancer cells in ovarian cancer, and is involved in the signaling pathways of CASP/PAK2-p34/actin. Cell Death Dis. 2014;5:e995. PMID: 24434518 DOI: 10.1038/cddis.2013.523
22. Zhang L, Jia G, Shi B, Ge G, Duan H, Yang Y. PRSS8 is Downregulated and suppresses tumour growth and metastases in hepatocellular carcinoma. Cell Physiol Biochem. 2016;40(3-4):757-69. PMID: 27915333 DOI: 10.1159/000453136
23. Keshamouni VG, Schiemann WP. Epithelial-mesenchymal transition in tumor metastasis: a method to the madness. Future Oncol. 2009;5(8):1109-11. PMID: 19852724 DOI: 10.2217/fon.09.87
24. Chen LM, Zhang X, Chai KX. Regulation of prostasin expression and function in the prostate. Prostate. 2004;59(1):1-12. PMID: 14991861 DOI: 10.1002/pros.10346

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Avicenna Journal of Clinical Medicine

Designed & Developed by : Yektaweb