Volume 30, Issue 2 (Avicenna Journal of Clinical Medicine-Summer 2023)
Avicenna J Clin Med 2023, 30(2): 106-113 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yarmohammadi E, Kasraei S, Danesh M H, Zarei F. Assessment of the Effect of 10% Trypsin and 25% Bromelain on the Micro-Shear Bond Strength of Etch-and-Rinse Adhesive Systems. Avicenna J Clin Med 2023; 30 (2) :106-113
URL: http://sjh.umsha.ac.ir/article-1-2722-en.html
URL: http://sjh.umsha.ac.ir/article-1-2722-en.html
1- Department of Restorative Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
2- Department of Restorative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Dentist
4- Department of Restorative Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran , zareifateme75@yahoo.com
2- Department of Restorative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Dentist
4- Department of Restorative Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran , zareifateme75@yahoo.com
Abstract: (552 Views)
Background and Objective: The removal of collagen by chemical methods can increase the micro-shear band strength of composite restorations. The present study aimed to investigate the effect of bromelain 25% and trypsin 10% on the micro-shear bond strength of Etch-and-rinse adhesives.
Materials and Methods: After cutting the chewing surface and exposing the dentin to a diameter of 5mm, the samples were randomly assigned to three equal groups (n=45): in the control group, after etching the adhesive surface, a Single Bond was used. In the other group, after etching, the surface was prepared with bromelain enzyme. In the last group, the trypsin enzyme was used to prepare the surface before applying the adhesive. Thereafter, the cylinders of ES Flow composite were placed on the bond surface. Following that, the micro-shear strength of the samples was calculated in megapascals.
Results: There was a significant difference between the mean of the trypsin group and that of the control group (P=0.001), as well as the bromelain group and the control group (P<0.001).
Conclusion: The preparation of etched dentin by trypsin and bromelain enzyme solutions increased the micro-shear bond strength between composite materials and dental dentin in Etch-and-rinse adhesives.
Materials and Methods: After cutting the chewing surface and exposing the dentin to a diameter of 5mm, the samples were randomly assigned to three equal groups (n=45): in the control group, after etching the adhesive surface, a Single Bond was used. In the other group, after etching, the surface was prepared with bromelain enzyme. In the last group, the trypsin enzyme was used to prepare the surface before applying the adhesive. Thereafter, the cylinders of ES Flow composite were placed on the bond surface. Following that, the micro-shear strength of the samples was calculated in megapascals.
Results: There was a significant difference between the mean of the trypsin group and that of the control group (P=0.001), as well as the bromelain group and the control group (P<0.001).
Conclusion: The preparation of etched dentin by trypsin and bromelain enzyme solutions increased the micro-shear bond strength between composite materials and dental dentin in Etch-and-rinse adhesives.
Type of Study: Original |
Subject:
Restoration Dentistry
Extended Abstract [HTML 26 KB] (40 Download)
References
1. Menon A, Ganapathy DM, Mallikarjuna AV. Factors that influence the colour stability of composite resins. Drug Invent. 2019;11(3):744-9.
2. Powers JM, Sakagauchi RL, Craig RG. Restorative dental materials. Mosby, Missouri; 2006.
3. Kowsari A, Seraj B, Pasdar N. An evaluation on shear strength of composite resin bonded to primary teeth dentin after Nd: YAG laser radiation. JDM. 2002;15(1):46-54.
4. Dautel-Morazin A, Vulcain J-M, Bonnaure-Mallet M. An ultrastructural study of the smear layer: comparative aspects using secondary electron image and backscattered electron image. J Endod. 1994;20(11):531-4. PMID: 7643035 DOI: 10.1016/S0099-2399(06)80066-X
5. Van Meerbeek B, Perdigao J, Lambrechts P, Vanherle G. The clinical performance of adhesives. J Dent. 1998;26(1):1-20. PMID: 9479920 DOI: 10.1016/s0300-5712(96)00070-x
6. Motamedi M, Alavi S, Abbasi M. In vitro comparison of the shear bond strength of “Etch & Rinse” vs “Self-Etch” bonding systems on dentin after “Nonvital Tooth Bleaching”. J Mashhad Dental School. 2010;34(3):237-46. DOI: 10.22038/JMDS.2010.1191
7. Gwinnett A, Yu S. Effect of long-term water storage on dentin bonding. Am J Dent. 1995;8(2):109-11. PMID: 7546480
8. . De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, et al. Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res. 2003;82(2):136-40. PMID: 12562888 DOI: 10.1177/154405910308200212
9. Dayem RN, Tameesh MA. A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system. Contemp Clin Dent. 2013;4(4):421-6. PMID: 24403782 DOI: 10.4103/0976-237X.123015
10. Zheng P, Chen H. Evaluate the effect of different mmps inhibitors on adhesive physical properties of dental adhesives, bond strength and mmp substarte activity. Sci Rep. 2017;7(1):1-11. DOI: 10.1038/s41598-017-04340-1
11. Matos AB, Trevelin LT, Silva BT, Francisconi-Dos-Rios LF, Siriani LK, Cardoso MV. Bonding efficiency and durability: current possibilities. Braz Oral Res. 2017;31:e57. PMID: 28902237 DOI: 10.1590/1807-3107BOR-2017.vol31.0057
12. Summitt JB, Robbins JW, Hilton TJ, Schwartz RS. Fundamentals of operative dentistry: a contemporary approach. Quintessence Publishing; 2006.
13. Osorio R, Erhardt MC, Pimenta LA, Osorio E, Toledano M. EDTA treatment improves resin-dentin bonds’ resistance to degradation. J Dent Res. 2005;84(8):736-40. PMID: 16040732 DOI: 10.1177/154405910508400810
14. Cecchin D, Farina AP, Galafassi D, Barbizam JV, Corona SA, Carlini-Júnior B. Influence of sodium hypochlorite and edta on the microtensile bond strength of a self-etching adhesive system. J Appl Oral Sci. 2010;18(4):385-9. PMID: 20835574 DOI: 10.1590/s1678-77572010000400011
15. Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol IL, Geraldeli S, et al. Strategies to prevent hydrolytic degradation of the hybrid layer—a review. Dent Mater. 2013;29(10):999-1011. PMID: 23953737 DOI: 10.1016/j.dental.2013.07.016
16. Reis A, Giannini M, Pereira P. Influence of water-storage time on the sorption and solubility behavior of current adhesives and primer/adhesive mixtures. Oper Dent. 2007;32(1):53-9. PMID: 17288329 DOI: 10.2341/06-13
17. Moszner N, Salz U, Zimmermann J. Chemical aspects of self-etching enamel–dentin adhesives: a systematic review. Dent Mater. 2005;21(10):895-910. PMID: 16038969 DOI: 10.1016/j.dental.2005.05.001
18. Gwinnett A. Altered tissue contribution to interfacial bond strength with acid conditioned dentin. Am J Dent. 1994;7(5):243-6. PMID: 7986445
19. Khan R, Sharma N, Garg Y, Kumar G, Garg K, Aleemuddin M. Comparison of different dentin deproteinizing agents on the shear bond strength of resin-bonded dentin. Int J Clin Pediatr Dent. 2020;13(1):69-77. PMID: 34434017 DOI: 10.5005/jp-journals-10005-1877
20. Kasraie S, Yarmohammadi E, Farhadian M, Malek M. Effect of bromelain and trypsin on microleakage of etch-and-rinse adhesive systems. J Stomatol. 2020;73(4):183-92.
21. Aguilera FS, Osorio R, Osorio E, Moura P, Toledano M. Bonding efficacy of an acetone/based etch-and-rinse adhesive after dentin deproteinization. Med Oral Patol Oral Cir Bucal. 2012;17(4):649-54. PMID: 22322501 DOI: 10.4317/medoral.17717
22. Chauhan K, Basavanna RS, Shivanna V. Effect of bromelain enzyme for dentin deproteinization on bond strength of adhesive system. J Conserv Dent. 2015;18(5):360-3. PMID: 26430297 DOI: 10.4103/0972-0707.164029
23. Abuhaimed TS, Abou Neel EA. Sodium hypochlorite irrigation and its effect on bond strength to dentin. BioMed research international. 2017;2017:1930360. PMID: 28904947 DOI: 10.1155/2017/1930360
24. Bellenger V, Verdu J, Morel E. Structure-properties relationships for densely cross-linked epoxide-amine systems based on epoxide or amine mixtures. J Mater Sci. 1989;24(1):63-8. DOI: 10.1007/BF00660933
25. Sharafeddin F, Safari M. Effect of papain and bromelain enzymes on shear bond strength of composite to superficial dentin in different adhesive systems. J Contemp Dent Pract. 2019;20(9):1077-81. PMID: 31797833
26. Siqueira F, Cardenas A, Gomes G, Chibinski A, Gomes O, Bandeca M, et al. Three-year effects of deproteinization on the in vitro durability of resin/dentin-eroded interfaces. Oper Dent. 2018;43(1):60-70. PMID: 29284095 DOI: 10.2341/16-308-L
27. Farina AP, Cecchin D, Vidal CM, Leme-Kraus AA, Bedran-Russo AK. Removal of water binding proteins from dentin increases the adhesion strength of low-hydrophilicity dental resins. Dent Mater. 2020;36(10):302-8. PMID: 32811665 DOI: 10.1016/j.dental.2020.07.004
28. Lisboa DS, Santos SV, Griza S, Rodrigues JL, Faria-e-Silva AL. Dentin deproteinization effect on bond strength of self-adhesive resin cements. Braz Oral Res. 2013;27(1):73-5. PMID: 23306629 DOI: 10.1590/s1806-83242013000100013
29. Pereira PNR, Bedran‐de‐Castro A, Duarte WR, Yamauchi M. Removal of noncollagenous components affects dentin bonding. J Biomed Mater Res B Appl Biomater. 2007;80(1):86-91. PMID: 16680689 DOI: 10.1002/jbm.b.30572
30. Bedran‐Russo AK, Pereira PN, Duarte WR, Okuyama K, Yamauchi M. Removal of dentin matrix proteoglycans by trypsin digestion and its effect on dentin bonding. J Biomed Mater Res B Appl Biomater. 2008;85(1):261-6. DOI: 10.1002/jbm.b.30944
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s).
Owned by Hamadan University of Medical Sciences
Published by UMSHA Press
Journal Tel: +988138380924
Publisher Tel:+988138380548
Website: http://sjh.umsha.ac.ir
Email: s_ journal[at]umsha.ac.ir
