Volume 28, Issue 1 (Avicenna Journal of Clinical Medicine-Spring 2021)                   Avicenna J Clin Med 2021, 28(1): 59-65 | Back to browse issues page


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Ghasemi Basir H R, Keramat F, Moradi A, Khani Z, Saadatmand A. Comparison of Antibiogram Results of Two Types of Antibiotic Discs and Validation by E.test Method on Bacteria with High Antibiotic Resistance Patterns. Avicenna J Clin Med. 2021; 28 (1) :59-65
URL: http://sjh.umsha.ac.ir/article-1-2205-en.html
1- Associate Professor, Department of Pathology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Professor, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
3- Instractor, Department of Community Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- General Practitioner, Hamadan University of Medical Sciences, Hamadan, Iran
5- MSc in Microbiology, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , Ali.s_umsha@yahoo.com
Abstract:   (524 Views)
Background and Objective: The increased resistance of microorganisms to common antibiotics is one of the major therapeutic challenges for patients. The present study aimed to compare the antibiogram results of two types of antibiotic discs and validate them by the Epsilometer test (E-test) method on bacteria with high antibiotic resistance patterns.
Materials and Methods: This descriptive-analytical study was performed by the census method on 176 patients admitted to different wards of Sina Hospital in Hamadan from March 2016 to March 2019. The patients entered the study with a positive culture result and underwent the antibiogram using two types of discs. Following that, validation was performed by the E-test method in cases with significant differences. Finally, the results were described and analyzed in SPSS software (version 16) at a 95% confidence interval.
Results: Out of 176 patients, 80 (45.4%) and 96 (54.5%) cases were male and female, respectively. The mean age of patients was reported as 62.44.±20.47 years. The most common culture sites were trachea (45.5%) and urine (42.6%), and the most common cultured microorganisms were E.coli (33.5%), Klebsiella (29.5%), and Acinetobacter (18.8%). The highest correlation coefficients of the results by disk diffusion method were observed in levofloxacin (r=1.00), piperacillin (r=0.917), ciprofloxacin (r=0.907), cotrimoxazole (r=0.904). On the other hand, the lowest correlation coefficients were related to imipenem (r=0.634), cefoxitin (r=0.556), and ceftriaxone (r=0.552).
Conclusion: As evidenced by the obtained results, significant differences were detected between the results of antibiogram in Iranian and imported disks. After verification and validation using E.test, the percentage of agreement was in favor of imported disks.
 
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Type of Study: Original | Subject: Pathology

References
1. Espinal MA, Laszlo A, Simonsen L, Boulahbal F, Kim SJ, Reniero A, et al. International :union: against tuberculosis and lung disease working group on anti-tuberculosis drug resistance surveillance. Global trends in resistance to antituberculosis drugs. N Engl J Med. 2001;344(17):1294-303. PMID: 11320389 DOI: 10.1056/NEJM200104263441706
2. Dadgari F, Ahmadi K, Mardani, Ramazan KA. Frequency and antibiotic resistance profile of bacteria isolated from the intensive care unit and General ward at a general hospital in Tehran. Ann Mil Health Sci Res. 2007;5(1):1155-64.
3. Keramat F, Homayoonfar S, Seif Rabiei M A, Abbasi K, Saadatmand A. Antibiotic resistance pattern of bacterial agents isolated from blood culture of patients with infective endocarditis. Tehran Univ Med J. 2019;77(4):240-5.
4. Van Hoek AH, Mevius D, Guerra B, Mullany P, Roberts AP, Aarts HJ. Acquired antibiotic resistance genes: an overview. Front Microbiol. 2011;2:203. PMID: 22046172 DOI: 10.3389/fmicb.2011.00203
5. Keshavarz-Hedayati S, Shapouri R, Habibollah-Pourzereshki N, Bigverdi R, Peymani A. Molecular investigation of resistance to disinfectants in acinetobacter baumannii isolates collected from Qazvin hospitals, Iran (2017). J Qazvin Univ Med Sci. 2019;23(1):2-13. DOI: 10.32598/JQUMS.23.1.2
6. Girish N, Saileela K, Mohanty S. Extended Spectrum β-Lactamase producing Klebsiella pneumoniae and Escherichia coli in neonatal intensive care unit. J Bacteriol Parasitol. 2012;3(4):e1000141. DOI: 10.4172/2155-9597.1000141
7. Poulou A, Grivakou E, Vrioni G, Koumaki V, Pittaras T, Pournaras S, et al. Modified CLSI extended-spectrum β-lactamase (ESBL) confirmatory test for phenotypic detection of ESBLs among Enterobacteriaceae producing various β-lactamases. J Clinl Microbiol. 2014;52(5):1483-9. PMID: 24574283 DOI: 10.1128/JCM.03361-13
8. Livermore DM. Of Pseudomonas, porins, pumps and carbapenems. J Antimicrobial Chemother. 2001;47(3):247-50. PMID: 11222556 DOI: 10.1093/jac/47.3.247
9. Majzoobi M M, Pirdehghan A, Rashidian Z, Saadatmand A. Etiologic agents and antibiotic resistance pattern of community- and hospital-acquired infections. Avicenna J Clin Med. 2018;25(1):41-8. DOI: 10.21859/ajcm.25.1.41
10. Moayeri A, Aminshokravi F, Tavafian S, Moayeri A. Assessing related factors on the illicit use of medications in Abbas Abad city(Mazandaran): a cross sectional study. J Ilam Univ Med Sci. 2014;22(5):11-9.
11. Coia JE, Duckworth GJ, Edwards DI, Farrington M, Fry C, Humphreys H, et al. Guidelines for the control and prevention of Meticillin-Resistant Staphylococcus aureus (MRSA) in healthcare facilities. J Hosp Infect. 2006;63(1):S1-44. PMID: 16581155 DOI: 10.1016/j.jhin.2006.01.001
12. Klevens RM, Edwards JR, Richards CL Jr, Horan TC, Gaynes RP, Pollock DA, et al. Estimating health care-associated infections and deaths in U.S. Hospitals, 2002. Public Health Rep. 2007;122(2):160-6. PMID: 17357358 DOI: 10.1177/003335490712200205
13. Luyt CE, Bréchot N, Trouillet JL, Chastre J. Antibiotic stewardship in the intensive care unit. Crit Care. 2014;18(5):480. PMID: 25405992 DOI: 10.1186/s13054-014-0480-6
14. Thuong M, Arvaniti K, Ruimy R, de la Salmonière P, Scanvic-Hameg A, Lucet JC, et al. Epidemiology of Pseudomonas aeruginosa and risk factors for carriage acquisition in an intensive care unit. J Hosp Infect. 2003;53(4) 274-82. PMID: 12660124 DOI: 10.1053/jhin.2002.1370
15. Odland BA, Erwin ME, Jones RN. Quality control guidelines for disk diffusion and broth microdilution antimicrobial susceptibility tests with seven drugs for veterinary applications. J Clin Microbiol. 2000;38(1):453-5. PMID: 10618141
16. Hanberger H, Nilsson L, Claesson B, Kärnell A, Larsson P, Rylander M, et al. New species-related MIC breakpoints for early detection of development of resistance among Gram-negative bacteria in Swedish intensive care units. J Antimicrobial Chemother. 1999;44(5):611-9. PMID: 10552977 DOI: 10.1093/jac/44.5.611
17. Fahimzad A, Mamaishi S, Noorbakhsh S, Siadati A, Hashemi F, Tabatabaei S, et al. Study of antibiotics resistance in pediatric acute bacterial meningitis with E-Test method. Iran J Pediatr. 2006;16(2):149-56.
18. Sedighi I, Solgi A, Alikhani MY, Emad Momtaz H, Mihani F. Comparison of two different disk diffusion agar tests in determination of antibiotic susceptibility for E-coli isolated from urinary tract infection in pediatrics. Avicenna J Clin Med. 2010;17(1):17-20.
19. Ahmadi A, Soltanpour MM, Imani Fooladi AA. Prevalence of imipenem-resistant bacterial strains isolated from hospital and accuracy of Iranian imipenem disc product. J Gorgan Univ Med Sci. 2015;17(1):61-6.
20. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty-fifth informational supplement. Wayne, PA: Clinical and Laboratory Standards Institute; 2017.
21. EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP). Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA J. 2012;10(6):2740. DOI: 10.2903/j.efsa.2012.2740
22. Erfani Y, Safdari R, Chobineh H, Mirsalehian A, Rasti A, Einollahi N, et al. Comparison of E. test and disk diffusion agar in detection of antibiotic susceptibility of E. coli isolated from patients with urinary tract infection in Tehran Shariati Hospital. Avicenna J Clin Med. 2008;15(2):27-31.

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