Volume 22, Issue 2 (Scientific Journal of Hamadan University of Medical Sciences-Summer 2015)                   Sci J Hamadan Univ Med Sci 2015, 22(2): 83-92 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ashengroph M, Haidarizadeh M, Borchaluei M. Use of Resting Cells of Native Screened Rhodotorula sp. CW03 in Biotransformation of Caffeine to Theophylline and Paraxanthine. Sci J Hamadan Univ Med Sci . 2015; 22 (2) :83-92
URL: http://sjh.umsha.ac.ir/article-1-37-en.html

Assistant Professor, Department of Biological Sciences and Biotechnology, School of Sciences, Kurdistan University, Sanandaj, Iran. , m.ashengroph@uok.ac.ir
Abstract:   (1480 Views)

Introduction & Objective: In recent years, microorganisms have been applied as biocatalysts for making pharmaceutically natural products. Microbial biotransformation of caffeine suggests a dual approach for biodegradation of toxic caffeine from polluted environments and a method for the production of medically and pharmaceutically valuable dimethylxanthines. The present work describes the identification of native yeasts capable of biotransformation of caffeine into theophylline and paraxanthine.

Materials & Methods: In this experimental study fourteen yeast strains which were able to de-grade caffeine isolated based on their morphology were selected as biocatalysts for biotrans-formation of caffeine as a low-cost substrate to high value added dimethylxanthines such as theophylline and theobromine. The selected strains were characterized based on phenotypic and genetic tests. Screening was performed by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) analyses.

Results: The results obtained using TLC and HPLC analyses suggest formation of two main metabolites of theophylline and paraxanthine from biotransformation of caffeine under resting cells of Rhodotorula sp. CW03 (GenBank accession number KF414531). The results showed that under resting cell conditions a maximum concentration of theophylline 380 mg/l (molar yield of 16.4%) and paraxanthine 880 mg/l (molar yield of 37.9%) were obtained after 72 h and 120 h of conversion time, respectively.

Conclusion: In the current investigation, done for the first time in Iran, we describe the isola-tion and identification of yeast strains with caffeine degradation ability which can be proposed as safe and cost-effective biocatalysts in production of value added dimethylxanthines from caffeine as a low-cost substrate.

Full-Text [PDF 549 kb]   (386 Downloads)    
Type of Study: Original | Subject: Special

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Scientific Journal of Hamadan University of Medical Sciences

Designed & Developed by : Yektaweb