Volume 28, Issue 2 (Avicenna Journal of Clinical Medicine-Winter 2021)                   Avicenna J Clin Med 2021, 28(2): 79-86 | Back to browse issues page


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Irani S, Alimohammadi S, Najafian T. Evaluation of the Expression Levels of Endothelin-1 and its Receptor (ETAR) in Dental Lamina during Different Stages of Development of Human Fetal Teeth. Avicenna J Clin Med. 2021; 28 (2) :79-86
URL: http://sjh.umsha.ac.ir/article-1-2246-en.html
1- Associate Professor, Department of Oral Pathology, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , irani@umsha.ac.ir
2- Associate Professor, Department of Obstetrics and Gynecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Dentist
Abstract:   (424 Views)
Background and Objective: Due to the significant importance of the teeth in mastication, speech, and aesthetics, it is necessary to identify all involved genes in the tooth development. Therefore, this study aimed to evaluate the role of endothelin-1 and its A receptor in dental lamina in different stages of tooth development.
Materials and Methods: This cross-sectional study included 33 fetal samples that were divided into three groups regarding gestational age. All samples were then stained by immunohistochemistry. Subsequently, the analysis was conducted in SPSS software (version 20) through the two-way ANOVA and Tukey's tests to examine the differences between the variables.  A p-value less than 0.05 was considered statistically significant.
Results: There was a significant difference between the gestational age and the expression level of endothelin-1 in dental lamina (P<0.001). In addition, a significant relationship was observed between age and anatomic area (P<0.001). There was also a significant difference between the gestational age and the expression level of endothelin-1 receptor (ETAR) in dental lamina (P<0.001). A significant association was found between gestational age and anatomic area (P<0.001).
Conclusion: The expression levels of endothelin-1 and its receptor (ETAR) in each jaw were higher in anterior dental lamina, compared to posterior dental lamina. These results may confirm the role of endothelin-1 and its receptor in cell proliferation, differentiation of dental lamina, and  calcium ion transport during tooth development
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Type of Study: Original | Subject: Oral Pathology

References
1. . Frisdal A, Trainor PA. Development and evolution of the pharyngeal apparatus. Wiley Interdiscip Rev Dev Biol. 2014;3(6):403-18. PMID: 25176500 DOI: 10.1002/wdev.147
2. . Nanci A. Ten cate's oral histology-e-book: development, structure, and function. NewYork: Elsevier; 2017.P. 185-217.
3. . Honda MJ, Fong H, Iwatsuki S, Sumita Y, Sarikaya M. Tooth-forming potential in embryonic and postnatal tooth bud cells. Med Mol Morphol. 2008;41(4):183-92. PMID: 19107607 DOI: 10.1007/s00795-008-0416-9
4. . Fraser GJ, Standing A, Underwood C, Thiery AP. The dental lamina: an essential structure for perpetual tooth regeneration in sharks. Integr Comp Biol. 2020;60(3):644-55. PMID: 32663287 DOI: 10.1093/icb/icaa102
5. . Guven G, Gunhan O, Akbulut E, Cehreli ZC. Investigation of proliferative activity in the developing human tooth using Ki-67 immunostaining. Med Princ Pract. 2007;16(6):454-9. PMID: 17917446 DOI: 10.1159/000107751
6. . Smith MM, Fraser GJ, Mitsiadis TA. Dental lamina as source of odontogenic stem cells: evolutionary origins and developmental control of tooth generation in gnathostomes. J Exp Zool B Mol Dev Evol. 2009;312B(4):260-80. PMID: 19156674 DOI: 10.1002/jez.b.21272
7. . Kawasaki K, Kawasaki M, Watanabe M, Idrus E, Nagai T, Oommen S, et al. Expression of Sox genes in tooth development. Int Dev Biol. 2015;59(10-12):471-8. PMID: 26864488 DOI: 10.1387/ijdb.150192ao
8. . Irani S, Salajegheh A, Smith RA, Lam AK. A review of the profile of endothelin axis in cancer and its management. Crit Rev Oncol Hematol. 2014;89(2):314-21. PMID: 24035584 DOI: 10.1016/j.critrevonc.2013.08.011
9. . Clouthier DE, Williams SC, Hammer RE, Richardson JA, Yanagisawa M. Cell-autonomous and nonautonomous actions of endothelin-A receptor signaling in craniofacial and cardiovascular development. Dev Biol. 2003;261(2):506-19. PMID: 14499656 DOI: 10.1016/s0012-1606(03)00128-3
10. . Pla P, Larue L. Involvement of endothelin receptors in normal and pathological development of neural crest cells. Int Dev Biol. 2003;47(5):315-25. PMID: 12895026
11. . Neuhaus SJ, Byers MR. Endothelin receptors and endothelin-1 in developing rat teeth. Arch Oral Biol. 2007;52(7):655-62. PMID: 17316550 DOI: 10.1016/j.archoralbio.2006.12.022
12. . Banerjee S, Mukherjee S, Nandini D, Sanjeeta N, Devi PA, Singhal P. Role of epithelial-mesenchymal transition in orofacial development-An insight. J Adv Clin Res Ins. 2019;6(3):83-5. DOI: 10.15713/ins.jcri.266
13. Liu M, Zhao L, Hu J, Wang L, Li N, Wu D, et al. Endothelial cells and endothelin‑1 promote the odontogenic differentiation of dental pulp stem cells. Mol Med Rep. 2018;18(1):893-901. PMID: 29845193 DOI: 10.3892/mmr.2018.9033
14. . Spath L, Rotilio V, Alessandrini M, Gambara G, De Angelis L, Mancini M, et al. Explant-derived human dental pulp stem cells enhance differentiation and proliferation potentials. J Cell Mol Med. 2010;14(6b):1635-44. PMID: 19602052 DOI: 10.1111/j.1582-4934.2009.00848.x
15. . Wyk LV, Smith J. Postnatal foot length to determine gestational age: a pilot study. J Trop Pediatr. 2016;62(2):144-51. PMID: 26758249 DOI: 10.1093/tropej/fmv093
16. . Irani S, Mohsenifar Z. Endocan, ET-1, and ETAR expression profiles in unicystic ameloblastoma, multicystic ameloblastoma, and ameloblastic carcinoma. Middle East J Cancer. 2019;10(3):167-74. DOI: 10.30476/MEJC.2019.78562
17. . Irani S, Salajegheh A, Gopalan V, Smith RA, Lam AK. Expression profile of endothelin 1 and its receptor endothelin receptor A in papillary thyroid carcinoma and their correlations with clinicopathologic characteristics. Ann Diagn Pathol. 2014;18(2):43-8. PMID: 24332749 DOI: 10.1016/j.anndiagpath.2013.11.001
18. . Kim KS, Arima Y, Kitazawa T, Nishiyama K, Asai R, Uchijima Y, et al. Endothelin regulates neural crest deployment and fate to form great vessels through Dlx5/Dlx6-independent mechanisms. Mech Dev. 2013;130(11-12):553-66. PMID: 23933587 DOI: 10.1016/j.mod.2013.07.005
19. . Sato T, Kurihara Y, Asai R, Kawamura Y, Tonami K, Uchijima Y, et al. An endothelin-1 switch specifies maxillomandibular identity. Proc Nat Acad Sci USA. 2008;105(48):18806-11. PMID: 19017795 DOI: 10.1073/pnas.0807345105
20. . Setkova J, Lesot H, Matalova E, Witter K, Matulova P, Misek I. Proliferation and apoptosis in early molar morphogenesis-voles as models in odontogenesis. Int J Dev Biol.2003;50(5):481-9. PMID: 16586349 DOI: 10.1387/ijdb.052067js
21. . Kero D, Novakovic J, Vukojevic K, Petricevic J, Govorko DK, Biocina-Lukenda D, et al. Expression of Ki-67, Oct-4, γ-tubulin and α-tubulin in human tooth development. Arch Oral Biol. 2014;59(11):1119-29. PMID: 25062118 DOI: 10.1016/j.archoralbio.2014.05.025
22. . Buchtová M, Stembírek J, Glocová K, Matalová E, Tucker AS. Early regression of the dental lamina underlies the development of diphyodont dentitions. J Dent Res. 2012;91(5):491-8. PMID: 22442052 DOI: 10.1177/0022034512442896
23. . Priya SP, Higuchi A, Fanas SA, Ling MP, Neela VK, Sunil P, et al. Odontogenic epithelial stem cells: hidden sources. Lab Invest. 2015;95(12):1344-52. PMID: 26367485 DOI: 10.1038/labinvest.2015.108

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