Volume 20, Issue 4 (Scientific Journal of Hamadan University of Medical Sciences-Winter 2014)                   Avicenna J Clin Med 2014, 20(4): 273-279 | Back to browse issues page

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Ghazanfari T, Rashidi H, Jalaei S, Alijani P. In Vitro Immunomodulatory Effect of R10 Fraction of Garlic on Viability and Production of TNF-? in CD8+ T Cells. Avicenna J Clin Med. 2014; 20 (4) :273-279
URL: http://sjh.umsha.ac.ir/article-1-111-en.html
, hosein_rashidi79@yahoo.com
Abstract:   (2137 Views)

Introduction & Objective: -cells, especially CD8+ T lymphocytes are the most important cells in anti-tumor response. Previously R10 fraction of garlic extract was reported as an immuno-modulator which induced an effective cellular immunity and Th1 responses. In this study the in vitro immunomodulatory effect of R10 on CD8+ T cells viability and production of TNF-α were evaluated.

 Materials & Methods: In this experimental study, using monoclonal antibodies attached to magnetic beads with isolating columns by magnetic bead method, CD8+ T cells from spleen cells of Balb/C mice were isolated. R10 fraction based on molecular weight was prepared using Ultra filtration. MTT assay was used to evaluate cell viability. TNF-α level was meas-ured in the supernatant of culture of CD8+ T cells by ELISA. Obtained data was compared and analyzed using Nonparametric Test and Keraskel & Wanny's Test tests..

 Results: The findings indicate that all dilutions of R10 fraction increased cell viability of CD8+ T cells in comparison with the negative control group and in the presence of ConA with dilution of 1:50 of R10 fraction significantly increased cell viability of CD8+ T Cells compared to ConA alone. Secretion of TNF-α significantly increased by all dilutions of R10 fraction.

Conclusion: These findings suggest that R10 fraction of garlic can be used as an Immuno-modulator drug candidate for induction of cellular Immunity in tumor therapy.

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Type of Study: Original | Subject: Other Clinical Specialties

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