Volume 25, Issue 2 (Avicenna Journal of Clinical Medicine-Summer 2018)
Avicenna J Clin Med 2018, 25(2): 85-91 |
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Nami Y, Ghiami Rad M, Farah Bakhsh H, Imeni N. Study of Antibiotic Resistance Pattern in Coagulase-negative Staphylococci strains Isolated from Clinical Specimens . Avicenna J Clin Med 2018; 25 (2) :85-91
URL: http://sjh.umsha.ac.ir/article-1-1750-en.html
URL: http://sjh.umsha.ac.ir/article-1-1750-en.html
1- MSc in Microbiology, Young Researchers and Elite Club, Marand Branch, Islamic Azad University, Marand, Iran , imeni.nazila94@gmail.com
Abstract: (5251 Views)
Background and Objective: Due to the emergence and development of antimicrobial resistance in coagulase-negative Staphylococci (CoNS), which is mainly a normal flora of the skin surface and mucous membrane of humans, and the limitation of therapeutic options, this study was aimed to investigate the antibiotic resistance pattern in CoNS strains isolated from clinical specimens.
Materials and Methods: In this cross-sectional descriptive study, a total of 44 isolates of coagulase-negative staphylococci were examined from clinical specimens of the patients using standard biochemical methods. Disc diffusion test was utilized to detect resistance to common antibiotics. Multiplex polymerase chain reaction (PCR) was employed to determine the frequency of resistance genes, namely mecA and vanA.
Results: The results of disc diffusion test showed that the isolates had the highest resistance rate to erythromycin as 88.64%; while the lowest resistance rate to meropenem was observed 4.55%. A molecular analysis indicated the presence of 18.18% of the mecA gene in the isolates; however no isolates containing vanA gene were observed.
Conclusion: Considering the frequency of mecA gene, results of antibiotic resistance pattern among coagulase-negative Staphylococci strains, and lack of any resistance observations to vancomycin by PCR, it is necessary to conduct more precise laboratory methods for the detection of antibiotic resistance to prevent resistance spread in this bacterium
Materials and Methods: In this cross-sectional descriptive study, a total of 44 isolates of coagulase-negative staphylococci were examined from clinical specimens of the patients using standard biochemical methods. Disc diffusion test was utilized to detect resistance to common antibiotics. Multiplex polymerase chain reaction (PCR) was employed to determine the frequency of resistance genes, namely mecA and vanA.
Results: The results of disc diffusion test showed that the isolates had the highest resistance rate to erythromycin as 88.64%; while the lowest resistance rate to meropenem was observed 4.55%. A molecular analysis indicated the presence of 18.18% of the mecA gene in the isolates; however no isolates containing vanA gene were observed.
Conclusion: Considering the frequency of mecA gene, results of antibiotic resistance pattern among coagulase-negative Staphylococci strains, and lack of any resistance observations to vancomycin by PCR, it is necessary to conduct more precise laboratory methods for the detection of antibiotic resistance to prevent resistance spread in this bacterium
Type of Study: Original |
Subject:
Other Clinical Specialties
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