Volume 27, Issue 4 (Avicenna Journal of Clinical Medicine-Winter 2021)
Avicenna J Clin Med 2021, 27(4): 201-210 |
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Amini M A, Khodadadi I, Tayebinia H, Talebi S S, Afshar B, Karimi J. Up-Regulation of TPT1-AS1 and SAMMSON and Down-Regulation of LINC00961 Long Non-Coding RNAs (lncRNAs) as Potential Tumor Markers in Gastric Cancer. Avicenna J Clin Med 2021; 27 (4) :201-210
URL: http://sjh.umsha.ac.ir/article-1-2152-en.html
URL: http://sjh.umsha.ac.ir/article-1-2152-en.html
Mohammad Amin Amini1 
, Iraj Khodadadi2 , Heidar Tayebinia2 , Sayed Saman Talebi3 , Behrouz Afshar3 , Jamshid Karimi4
, Iraj Khodadadi2 , Heidar Tayebinia2 , Sayed Saman Talebi3 , Behrouz Afshar3 , Jamshid Karimi4
1- MSc in Clinical Biochemistry, Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Professor, Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Assistant Professor, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- Associate Professor, Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ,jamshidkarimi2013@gmail.com
2- Professor, Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Assistant Professor, Department of Internal Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- Associate Professor, Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ,
Abstract: (2821 Views)
Background and Objective:Gastric Cancer (GC) is one of the deadliest cancers in the world. Recently, LINC00961, TPT1-AS1, and SAMMSON Long non-coding RNA (lncRNAs)have been discovered, which significantly contribute to the occurrence of various cancers. This study aimed to determine the expression levels of these genes in GC tissues, compared to healthy adjacent tissues, and the relationship of their expressions with clinical characteristics.
Materials and Methods:In this case-control study, the expression of LINC00961, SAMMSON, and TPT1-AS1 were evaluated in 40 pairs of cancerous and adjacent non-cancerous tissue samples in GC patients using qRT-PCR. Statistical analysis and graphing of the relationship between RNA levels and the clinic-pathological characteristics of GC were carried out using SPSS and Prism 5.00 software. Furthermore, Receiver Operating Characteristic (ROC) curve was drawn to represent the sensitivity and specificity of LINC00961, SAMMSON, and TPT1-AS1 expression as biomarkers of GC.
Results:The expression of TPT1-AS1 and SAMMSON were significantly up-regulated, and the expression of LINC00961 was significantly decreased in GC specimens, compared to adjacent control samples. Our results showed that TPT1-AS1 and SAMMSON RNA levels in GC were significantly related to the tumor size and histopathological grade. Moreover, the ROC curve analysis of LINC00961 and SAMMSON RNA level demonstrated that these two lncRNAs had an appropriate sensitivity and specificity for the diagnosis goals.
Conclusion:Based on the results, LINC00961, SAMMSON, and TPT1-AS1 may play critical roles in exacerbating and even initiating GC due to their cellular pathways.
Materials and Methods:In this case-control study, the expression of LINC00961, SAMMSON, and TPT1-AS1 were evaluated in 40 pairs of cancerous and adjacent non-cancerous tissue samples in GC patients using qRT-PCR. Statistical analysis and graphing of the relationship between RNA levels and the clinic-pathological characteristics of GC were carried out using SPSS and Prism 5.00 software. Furthermore, Receiver Operating Characteristic (ROC) curve was drawn to represent the sensitivity and specificity of LINC00961, SAMMSON, and TPT1-AS1 expression as biomarkers of GC.
Results:The expression of TPT1-AS1 and SAMMSON were significantly up-regulated, and the expression of LINC00961 was significantly decreased in GC specimens, compared to adjacent control samples. Our results showed that TPT1-AS1 and SAMMSON RNA levels in GC were significantly related to the tumor size and histopathological grade. Moreover, the ROC curve analysis of LINC00961 and SAMMSON RNA level demonstrated that these two lncRNAs had an appropriate sensitivity and specificity for the diagnosis goals.
Conclusion:Based on the results, LINC00961, SAMMSON, and TPT1-AS1 may play critical roles in exacerbating and even initiating GC due to their cellular pathways.
Type of Study: Original |
Subject:
Clinical Biochemistry
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