Volume 32, Issue 2 (Avicenna Journal of Clinical Medicine-Summer 2025)
Avicenna J Clin Med 2025, 32(2): 80-86 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shokri S, Azizi Jalilian F, Amini R, Shojaeian A, Torkaman Asadi F, Mahmoudvand S. Down-regulation of Tumor Suppressor Gene PTEN in Patients with Chronic Hepatitis B Virus Infection. Avicenna J Clin Med 2025; 32 (2) :80-86
URL: http://sjh.umsha.ac.ir/article-1-3243-en.html
URL: http://sjh.umsha.ac.ir/article-1-3243-en.html
Somayeh Shokri1 
, Farid Azizi Jalilian2 , Razieh Amini3 , Ali Shojaeian3 , Fatemeh Torkaman Asadi4 , Shahab Mahmoudvand5
, Farid Azizi Jalilian2 , Razieh Amini3 , Ali Shojaeian3 , Fatemeh Torkaman Asadi4 , Shahab Mahmoudvand5
1- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran & Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran
2- Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran & Department of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran
4- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran & Department of Infectious Diseases, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
5- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran & Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran ,Mahmoudvandsh100@gmail.com
2- Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran & Department of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran
4- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran & Department of Infectious Diseases, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
5- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran & Research Center for Molecular Medicine, Institute of Cancer, Hamadan University of Medical Sciences, Hamadan, Iran ,
Abstract: (148 Views)
Background and Objective: Chronic Hepatitis B virus (HBV) infection is a major global health concern and a leading cause of hepatocellular carcinoma (HCC). Loss or suppression of PTEN expression contributes to the development and progression of HCC, particularly in the context of chronic HBV infection. The present study aimed to evaluate PTEN gene expression in patients with chronic HBV infection compared to healthy individuals.
Materials and Methods: The present case-control study included 50 patients with chronic HBV infection and 50 healthy controls. Peripheral blood samples were collected, and total RNA was extracted and converted to cDNA. Gene expression of PTEN relative to GAPDH was assessed by SYBR Green-based Real-Time PCR using the 2-ΔΔCt method. Statistical analysis was performed using the SPSS (version 26) software, with a significance set at P<0.05.
Results: The PTEN gene expression was significantly down-regulated in the HBV-infected group compared to controls (2-ΔΔCt = 0.074, P<0.0001). The ΔCt value of PTEN was significantly higher in the patient group, indicating reduced transcript levels. An age-related decline in PTEN expression was also observed among HBV patients.
Conclusion: The results highlight the importance of PTEN as a potential biomarker in the progression of the disease to liver cancer. Therefore, further research with a larger sample size and investigation of the molecular mechanisms involved in regulating PTEN expression seems necessary.
Materials and Methods: The present case-control study included 50 patients with chronic HBV infection and 50 healthy controls. Peripheral blood samples were collected, and total RNA was extracted and converted to cDNA. Gene expression of PTEN relative to GAPDH was assessed by SYBR Green-based Real-Time PCR using the 2-ΔΔCt method. Statistical analysis was performed using the SPSS (version 26) software, with a significance set at P<0.05.
Results: The PTEN gene expression was significantly down-regulated in the HBV-infected group compared to controls (2-ΔΔCt = 0.074, P<0.0001). The ΔCt value of PTEN was significantly higher in the patient group, indicating reduced transcript levels. An age-related decline in PTEN expression was also observed among HBV patients.
Conclusion: The results highlight the importance of PTEN as a potential biomarker in the progression of the disease to liver cancer. Therefore, further research with a larger sample size and investigation of the molecular mechanisms involved in regulating PTEN expression seems necessary.
Type of Study: Original |
Subject:
Infectious Diseases
Extended Abstract [HTML 26 KB] (19 Download)
References
1. Nahid Samiei R, Shokri S, Mahmoudvand S, Makvandi M, Shahbazian H, Shayanpur S, et al. Occult hepatitis B virus infection among hemodialysis patients. J Biol Res Bollettino della Società Italiana di Biol Sperimentale. 2018;91(2). DOI:10.4081/jbr.2018.7516
2. Al-Busafi SA, Alwassief A. Global perspectives on the hepatitis b vaccination: challenges, achievements, and the road to elimination by 2030. Vaccines (Basel). 2024;12(3):228. PMID: 38543922 DOI: 10.3390/vaccines12030288
3. Sheikhrobat SB, Mahmoudvand S, Kazemipour-Khabbazi S, Ramezannia Z, Baghi HB, Shokri S. Understanding lactate in the development of Hepatitis B virus-related hepatocellular carcinoma. Infect Agent Cancer. 2024;19(1):31. PMID: 39010155 DOI: 10.1186/s13027-024-00593-4
4. . Kim DY. Changing etiology and epidemiology of hepatocellular carcinoma: Asia and worldwide. J Liver Cancer. 2024;24(1):62-70. PMID: 38523466 DOI: 10.17998/jlc.2024.03.13
5. Agustiningsih A, Rasyak MR, Turyadi, Jayanti S, Sukowati C. The oncogenic role of hepatitis B virus X gene in hepatocarcinogenesis: recent updates. Explor Target Antitumor Ther. 2024;5(1):120-34. PMID: 38464387 DOI: 10.37349/etat.2024.00209
6. Wang Q, Wang J, Xiang H, Ding P, Wu T, Ji G. The biochemical and clinical implications of phosphatase and tensin homolog deleted on chromosome ten in different cancers. Am J Cancer Res. 2021;11(12):5833-55. PMID: 35018228
7. Ertay A, Ewing RM, Wang Y. Synthetic lethal approaches to target cancers with loss of PTEN function. Genes Dis. 2023;10(6):2511-27. PMID: 37533462 DOI: 10.1016/j.gendis.2022.12.015
8. Chang H, Cai Z, Roberts TM. The mechanisms underlying PTEN loss in human tumors suggest potential therapeutic opportunities. Biomolecules. 2019;9(11):713. PMID: 31703360 DOI: 10.3390/biom9110713
9. Chung TW, Lee YC, Ko JH, Kim CH. Hepatitis B Virus X protein modulates the expression of PTEN by inhibiting the function of p53, a transcriptional activator in liver cells. Cancer Res. 2003;63(13):3453-8. PMID: 12839924
10. Sun Y, Yu M, Qu M, Ma Y, Zheng D, Yue Y, et al. Hepatitis B virus-triggered PTEN/β-catenin/c-Myc signaling enhances PD-L1 expression to promote immune evasion. Am J Physiol Gastrointest Liver Physiol. 2020;318(1):G162-73. PMID: 31604033 DOI: 10.1152/ajpgi.00197.2019
11. Eskandari E, Dahmardeh T, Dahmardeh F, Pahlevani E, Metanat M. Lack of relationship between PTEN 32-bp and TP53 16-bp Ins/Del polymorphisms and chronic hepatitis B virus infection. Virusdisease. 2017;28(3):289-94. PMID: 29291215 DOI: 10.1007/s13337-017-0391-7
12. . Ma S, Qin K, Ouyang H, Zhu H, Lei P, Shen G. HBV infection exacerbates PTEN defects in hepatocellular carcinoma through upregulation of miR-181a/382/362/19a. Am J Transl Res. 2020;12(7):3780-91. PMID: 32774734
13. Loomba R, Friedman SL, Shulman GI. Mechanisms and disease consequences of nonalcoholic fatty liver disease. Cell. 2021;184(10):2537-64. PMID: 33989548 DOI: 10.1016/j.cell.2021.04.015
14. El-Serag HB. Epidemiology of viral hepatitis and hepatocellular carcinoma. Gastroenterology. 2012;142(6):1264-73.e1. PMID: 22537432 DOI: 10.1053/j.gastro.2011.12.061
15. Du Y, Zhang YW, Pu R, Han X, Hu JP, Zhang HW, et al. Phosphatase and tensin homologue genetic polymorphisms and their interactions with viral mutations on the risk of hepatocellular carcinoma. Chin Med J (Engl). 2015;128(8):1005-13. PMID: 25881591 DOI: 10.4103/0366-6999.155057
16. Huang F, Guo J, Zhao N, Hou M, Gai X, Yang S, et al. PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1. J Transl Med. 2024;22:254. PMID: 38459588 DOI: 10.1186/s12967-024-04976-4
17. Tu W, Yang Y, Song Y, Zhu W. Hepatitis B virus x protein accelerated the proliferation of hepatocellular carcinoma cell through lncRNA SNHG20/PTEN pathway. J Biochem. 2019;165(5):423-31. PMID: 30690477 DOI: 10.1093/jb/mvy120
18. Zhu X, Zhang H, Xu J. Long noncoding RNA SNHG20 regulates cell migration, invasion, and proliferation via the microRNA-19b-3p/RAB14 axis in oral squamous cell carcinoma. Bioengineered. 2021;12(1):3993-4003. PMID: 34282711 DOI: 10.1080/21655979.2021.1950278
19. Ruan Y, Jiang Y, Wang W, Ma J, He B, Chen M. HBV down-regulates PTEN expression via Nrf2/GSK3β signaling pathway. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2020;45(9):1009-14. PMID: 33051413 DOI: 10.11817/j.issn.1672-7347.2020.200189
20. Kang-Park S, Im JH, Lee JH, Lee YI. PTEN modulates hepatitis B virus-X protein induced survival signaling in chang liver cells. Virus Res. 2006;122(1):53-60. PMID: 16872708 DOI: 10.1016/j.virusres.2006.06.010
21. Tian Y, Xiao X, Gong X, Peng F, Xu Y, Jiang Y, et al. HBx promotes cell proliferation by disturbing the cross-talk between miR-181a and PTEN. Sci Rep. 2017;7:40089. PMID: 28053323 DOI: 10.1038/srep40089
22. Tang J, Xiao X, Jiang Y, Tian Y, Peng Z, Yang M, et al. miR-3 encoded by hepatitis b virus downregulates pten protein expression and promotes cell proliferation. J Hepatocell Carcinoma. 2020;7:257-69. PMID: 33154957 DOI: 10.2147/JHC.S271091
23. Liu Y, Wang J, Chen J, Wu S, Zeng X, Xiong Q, et al. Upregulation of miR-520c-3p via hepatitis B virus drives hepatocellular migration and invasion by the PTEN/AKT/NF-κB axis. Mol Ther Nucleic Acids. 2022;29:47-63. PMID: 35795482 DOI: 10.1016/j.omtn.2022.05.031
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s).
Owned by Hamadan University of Medical Sciences
Published by UMSHA Press
Journal Tel: +988138380924
Publisher Tel:+988138380548
Website: http://sjh.umsha.ac.ir
Email: s_ journal[at]umsha.ac.ir
