Introduction & Objective: Fusariosis is one of the most important systemic mycosis, often caused by Fusarium Solani and resist to antifungal drugs. The appropriate F. Solani antigen preparation could be useful in serodiagnosis of fusariosis. 

Materials & Methods : The extraction procedure was preformed using F.Solani strain 7419 UAMH. The antigenic extract was obtained through grinding of fungal mass yielding from broth culture medium.

Results : Following fractionation of somatic antigen, two different component, that is, crude antigen as well as antigenic fractions (12,28) were collected.

The antigenic fractions in comparison with the crude antigen, demonstrated more effective antibody responses using ELISA method.

Conclusion: Availability of a suitable antigenic source could play a key role for serologic detecting of opportunistic fungal disease including fusariosis. Injection of this antigenic preparation in Rabbit resulted antibody response.