Volume 10, Issue 2 (Scientific Journal of Hamadan University of Medical Sciences-Summer 2003)                   Sci J Hamadan Univ Med Sci 2003, 10(2): 36-41 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hassanzadeh Ghasabeh T. Interrelationship between Manganese and Iron for Binding to Apo-Transferrin. Sci J Hamadan Univ Med Sci. 2003; 10 (2) :36-41
URL: http://sjh.umsha.ac.ir/article-1-670-en.html
Abstract:   (777 Views)

Manganese is an essential trace elenent. There is a little evidence for deficiency of manganese in human , while as its toxicity has been reported in several cases. Manganese toxicity occurrs in humans exposed to high enviromental concentrations (for example workers in the dry battery production) and may be particularly important in the neonatal period. The chemical similarities between manganese and iron and their binding to
   apo-transferrin (apo-tf) may lead to the disturbances of iron metabolism.

          In the present project the interrelationship of anemia and manganese toxicity by techniques such as Affinity chromatography ; Equilibrium dialysis ; Urea polyacrylamide gel electrophoresis ; Spectrophotometric titration was investigated.

          The charactristics of Mn and Fe binding to apo-tf have been investigated and compared in this article . Using Equilibrium dialysis technique the binding of Fe and Mn to apo-tf was also studied. The binding constant of Mn to apo-tf was calculated using scatchared plot analysis. Addition of Mn (1.5 mg/ml) to reaction mixture containing Fe and apo-tf reduced Fe binding to apo-transferrin in comparison to control. Using Urea polyacrylamide gel electrophoresis (Urea-PAGE) technique conforming the binding of Mn to apo-tf.

          The data that has been presented in this article elucidated the probable mechanism by which Mn interference with Fe metabolism; which result in the apperance of anemia.

Full-Text [PDF 239 kb]   (431 Downloads)    
Type of Study: Original | Subject: Special

Add your comments about this article : Your username or Email:
Write the security code in the box

© 2015 All Rights Reserved | Avicenna Journal of Clinical Medicine

Designed & Developed by : Yektaweb