Phenanthrolines are a group of organic compounds which can transfer metal ions through the plasma membranes of the cell. Due to their ionophoric characteristics, phenanthrolines are widely used in chemical and boilogical studies. In this research, the effect a new chelating agent 2,6 diaminopyridinum(1,10-Phenanthroline-2,9- dicarboxylate) whichwas synthesized in organic chemistry laboratory of Teacher Training University of  Tehran city , on the pituitary – gonad axis was studied.

          It was decided to find out the effects of this compound on the pituitary- gonad axis and testis tissue of adult Balb/C mice. LD₅₀ standard was found 35mg/kg B.W. Some doses of 15, 20 and 25 mg/kg of body weight were injected as sublethal doses of compound and continued for 20 days  intraperitonealy, while the control groups received the solvent (normal saline).

          The results showed that 25 mg/kg B.W. of the compound decreases testosterone level in the blood serum significantly(68.5%) but no significant changes were obtained in LH and FSH levels in experimental and control groups. Also low doses of 15 and 20 mg/kg B.W did not change the hormonal levels significantly. Histological investigations on the testis tissue showed that the number of sperm cells in doses of 15, 20 and 25 mg/kg B.W
   decreased 20.2% , 52.1% and 95.2% and did not showed any harmful side effects on the animals.

          According to the results of this study, it may concluded that this
   phenanthroline derivative in high dose decrease the level of blood serum
   testosterone and spermatogenesis.